Research Article Volume 5 Issue 1 - March 2018 DOI: 10.19080/GJPPS.2018.05.555655 Glob J Pharmaceu Sci Copyright © All rights are reserved by Savita Dixit Investigation of Immunomodulation Activity in the Leaves of Dalbergia dissoo Savita Dixit* and Shikha Rana Maulana Azad National Institute of Technology, India Submission: January 22, 2017; Published: March 01, 2018 *Corresponding author: Savita Dixit, Maulana Azad National Institute of Technology, India, Email: Glob J Pharmaceu Sci 5(1): GJPPS.MS.ID.555655 (2018) 001 Global Journal of Pharmacy & Pharmaceutical Sciences ISSN: 2573-2250 Introduction Medicinal plants have a compelling role in modern and ancient cultures. Diverse active principles have been originated from the conventional medicinal plants. WHO evaluates that 70% of humans still depends on the herbal medication. India is a rich source of herbal flora and medical systems used here are ancient [1]. The Indian medicinal systems are categorized into classical and traditional systems. These systems cover adapted systems and Indian originated systems which include Ayurveda, Siddha, Unani, Yoga and Naturopathy [2]. Ayurveda principally uses plant based formulations for treatment [3]. The synthesis of aromatic substance by plants is nearly inexhaustible. Most of the substances isolated are secondary metabolites which are estimated to be less than 10% of the total [4]. Very few of these metabolites have been investigated phytochemically. The percentage submitted to biological and pharmacological screening is even lower. As plants have myriad of metabolites many researchers are turning their concerns into medicinal plant research as it is the most emerging source for possible new lead compounds which can then be taken into therapeutically screening programmes. Plant based drugs are an important source of therapeutic agents because of their abundant availability, relatively cheaper cost and non-toxic nature when compared to modern medicine [5]. There is an enormous demand for the medicinal plants. Antioxidant property of various plants is being investigated because of the health benefits. An antioxidant is a molecule which is efficient in slowing or preventing the oxidation of other molecules [6]. Oxidation is a chemical reaction that transfers electron from a substance to an oxidizing agent. Oxidation reactions can produce free radicals, which start chain reactions that damage cells [7]. Antioxidants cease these reactions by removing the intermediates [8]. The modulation of immune system using plant products as a therapeutic measure is serving as an active scientific investigation. The most ancient of such practices are found in the Vedic scriptures. Immunomodulation could provide an alternative to conventional therapeutics under the conditions of impaired immune responsiveness. This concept also generates credibility when it is found that herbal antioxidants concurrently Abstract During the proper functioning of the immune system reactive oxygen or nitrogen species are generated. These can affect by inducing oxidative damage. This condition occurs with ageing and with diseases. This condition can be annihilated by introducing the defaces by antioxidants. Dietary supplementation with antioxidants can prove to be a stepping stone in these conditions. Natural compounds from plants having rich sources of antioxidants can act as immunomodulators. Aim: The aim of the present study is to investigate the immunomodulation activity in the extracts of Dalbergia Sissoo leaves. This includes in vitro testing of D. Sissoo leaves methanolic extract for its antioxidant activity and phytochemicals. Material and methods: The leaves of D. Sissoo are collected from a Sai nursery situated near Manisha market in Bhopal. These leaves were then washed and the disinfected and were then shade dried for 2 weeks and then grinded in the grinder. After grinding these were taken for extraction. The extraction was done by Soxhlet Method. The tests for antioxidant activity includes DPPH Scavenging activity, NO Scavenging activity, total antioxidant activity and phytochemical tests were performed according to standard methods as proposed by Harborne. Results and conclusion: The extract have excellent amount of flavonoids and phenolic contents which agrees to the rich antioxidant activity in the leaves extract. Various photochemical are also present in the extract. It is found that these leaves extract are excellent source of antioxidants and can be used as potent immunomodulator. Keywords: Immunomodulation; Antioxidant activity; D. Sissoo Leaves; DPPH scavenging; NO scavenging; Total antioxidant activity