220 2 2 946 Board #207 May 30 2:00 PM - 3:30 PM Effects of Acute Exercise and Green Tea Supplementation on Glucose Homeostasis in Overweight/Obese Postmenopausal Women Shannon L. Jordan 1 , Sarah E. Deemer 2 , Vic BenEzra 3 , David Nichols, FACSM 3 . 1 Lamar University, Beaumont, TX. 2 The University of Alabama at Birmingham, Birmingham, AL. 3 Texas Woman’s University, Denton, TX. (Sponsor: David Nichols, FACSM) (No relevant relationships reported) Menopause is associated with decreased estrogen levels, increased adiposity, negative changes in adiponectin and TNF-α, and increased insulin resistance. Decreased adiponectin and increased TNF-α are associated with impaired glucose uptake. Exercise enhances glucose uptake several hours post-exercise. Polyphenols in green tea extract (GTE) increase insulin sensitivity and adiponectin while decreasing TNF-α. Orzechowski (2003) proposed using antioxidants as a “preconditioning” method to prevent development of Type 2 Diabetes (T2D). PURPOSE: To investigate independent and combined effects of acute exercise or GTE on glucose homeostasis and adipokines in overweight to obese postmenopausal sedentary women. METHODS: Eight women (52 ± 7 yrs, BMI 32.04 ± 4.95 kg/m 2 ) were randomly assigned to complete four trials (Control [C], green tea [GT], exercise [EX], green tea + exercise [GTEX]). For each trial the participant consumed 400 mg of placebo (rice flour, C and EX) or green tea extract (GT and GTEX) with lunch and dinner the day prior and the morning of (1 hr prior) an oral glucose tolerance test (OGTT). Exercise trials (EX and GTEX) consisted of walking exercise at 65% heart rate reserve (400 Kcal) and were completed 12-14 hours prior to the OGTT. RESULTS: Fasting glucose (C 5.7 ±.8, GT 6.1 ± 1.7, EX 5.6 ± .8, GTEX 5.4 ± .8 mmol) and insulin (C 11.93 ± 4.75, GT 11.66 ± 6.17, EX 11.31 ± 4.12, GTEX 12.23 ±6.05μU/ml) were not different between trials as well as no differences in area under the curve for both glucose and insulin. There were also no differences between trials for TNF-α (C 5.45 ± 3.68, GT 4.73 ± 1.38, EX 3.55 ± 1.46, GTEX 3.87 ± 1.81 pg/ml) or total adiponectin (C 7443 ± 2941, GT 4497 ± 3070, EX 10060 ± 7393, GTEX 5335 ± 3193 ng/ml). DISCUSSION: An acute bout of exercise or green tea supplementation may not be sufficient to see a favorable impact in glucose homeostasis or adipokines within this population. Elevated TNF-α (> 1.36 pg/ml) and low adiponectin levels observed in all trials may reflect an inflammatory state that could be associated with menopause. Inflammation is known to alter glucose metabolism. Postmenopausal woman present a unique challenge with prevention of T2D, as increased adiposity and decreased estrogen levels negatively effects adipokines, which negatively impacts glucose homeostasis. 947 Board #208 May 30 2:00 PM - 3:30 PM Family History of Diabetes Does Not Affect Exercise-Induced Improvements in Insulin Sensitivity and Metabolic Flexibility Manuel Amador 1 , Cesar Meza 1 , Mario Garcia 1 , Christopher Figueroa 1 , George King, FACSM 1 , Andrew McAinch 2 , Sudip Bajpeyi 1 . 1 Universtiy of Texas at El Paso, El Paso, TX. 2 Victoria University, St. Albans Campus, Melbourne, Australia. (Sponsor: George King, FACSM) (No relevant relationships reported) A family history of diabetes (FH+) is considered a risk factor to develop insulin resistance and type 2 diabetes. However, it is not known whether exercise induced improvement in insulin sensitivity (IS) and metabolic flexibility (MF) are impacted by a FH+, compared to those without (FH-). PURPOSE: To determine if a FH+ limits exercise induced improvements in IS, MF, body composition, and strength following an 8-week combined aerobic and resistance training intervention. METHODS: 19 sedentary, normoglycemic, Mexican-American males underwent 8 weeks of combined exercise training 3 times/week (35-min aerobic & 45-min resistance training/session). A controlled diet was provided 5 days before pre/post intervention tests. IS was assessed by hyperinsulinemic euglycemic clamp. MF was assessed by change in respiratory quotient (ΔRQ) at the insulin stimulated state of the clamp compared to the fasted state. Body composition was me asured using DXA. Upper/lower body strength were measured by 1 repetition maximum bench press and leg strength dynamometer. RESULTS: IS significantly improved in both groups (FH- 2.95±31 to 3.84±0.31ml/kg estimated mean body size (EMBS), p=0.03; FH+ 3.6±0.5 to 4.8±0.5ml/kg EMBS; p=0.002). MF significantly improved in both groups (FH- 0.72±0.009 to 0.78±0.008, p=0.001; FH+ 0.70±0.01 to 0.80±0.02, p=0.0001). Fat free mass significantly improved in both groups (FH- 56.6±2.1 to 58.5±2.1kg, p=0.01; FH+ 51.8±1.95 to 53.4±1.79kg, p=0.01). Upper body strength (FH- 172.78±20.95 to 200.56±20.74lb, p=0.0001; FH+ 136.5±14.06 to 168.33±15.29lb, p=0.0001) and lower body strength (FH- 368.89±31.02 to 431.11±22.58lb, p=0.007; FH+ 341.0±22.03 to 412.78±16.22lb, p=0.0001) significantly increased in both groups. Degrees of improvement in IS was not different between groups (FH- 28.3±13.6% vs. FH+ 41.66±11.9%; p>0.05). CONCLUSION: FH+ is not a limiting factor for exercise induced improvements in IS, MF, body composition, and strength in normoglycemic Mexican Americans. 948 Board #209 May 30 2:00 PM - 3:30 PM Physical Activity and Glycemic Control in Low Versus High Inflammation Phenotypes in Metabolically Healthy Adults Morgan Chamberlin, Jamie Ritter, Adam Maes, Stephanie Wilson, Sarah Bronsky, Seth Walk, Carl Yeoman, Mary P Miles, FACSM. Montana State University, Bozeman, MT. (No relevant relationships reported) Metabolically healthy overweight or obese (MHO) individuals may be studied to better understand the relationship of low-level inflammation to physical activity (PA) and fitness, metabolic syndrome, glycemic control, and postprandial responses. It was hypothesized that individuals with lower inflammation would have greater volume of PA, cardiorespiratory fitness (VO 2 max), and metabolic health. PURPOSE: To compare PA, VO 2 max, glycosylated hemoglobin (HbA1c), metabolic syndrome criteria, and metabolic responses to a high-fat meal between low (LO) and high (HI) inflammation phenotypes within a group of MHO adults. METHODS: Adults (n=25), 23-54 y of age with BMI from 27-35 kg·m -2 were assessed for body composition, self-reported PA, VO max, and fasting/resting concentrations of interleukin (IL)-1β, IL-6, IL17-, IL-23, tumor necrosis factor-α (TNF-α), and granulocyte-macrophage colony stimulating factor (GM-CSF). LO (n=11) were below the group median for ≥ 4 of the cytokines; HI (n=12) above for ≥ 4. Two participants were between phenotypes. After fasting blood collection, a high fat meal (50 g fat, 54 g carbohydrate, and 12 g protein) was consumed and 4 hourly, postprandial blood samples were collected for measurement of glucose, insulin, and triglycerides (TG). RESULTS: Mean cytokine concentrations were 1.8 to 4.3-fold higher (p<0.05) in HI compared to LO for IL-1β, IL-6, IL17-, IL-23, TNF-α, but not (p=0.12) GM-CSF. The frequency of aerobic activity was higher (p<0.05; mean ± SEM; 5.2 ± 0.5 vs 3.1 ± 0.4 d·wk -1 ) while VO max was similar (p=0.21; 42.9 ± 2.7 vs 38.3 ± 2.2 ml·kg·min -1 ) for LO vs HI. Contrary to our hypothesis, LO were higher (p<0.05) in HbA1c (5.38 ± 0.08 vs 5.10 ± 0.07%) and 1-h postprandial glucose (116.6 ± 5.7 vs 102.1 ± 3.5 mg·dl -1 ). TG and insulin responses, BMI, body fat (%), visceral adipose (l), and metabolic syndrome criterion scoring for waist circumference, blood pressure, and fasting TG, glucose and HDL were similar between groups. CONCLUSION: The current data support the beneficial influence of physical activity on inflammation; however, the unexpected finding of healthier glycemic control in individuals with higher inflammation warrants additional research. Funding Acknowledgement: Montana University System Research Initiative 51040-MUSRI2015-03 and USDA-NIFA 2017-67018-26367. Copyright © 2018 by the American College of Sports Medicine. Unauthorized reproduction of this article is prohibited. Copyright © 2018 by the American College of Sports Medicine. Unauthorized reproduction of this article is prohibited.