CHEMICAL ENGINEERINGTRANSACTIONS VOL. 47, 2016 A publication of The Italian Association of Chemical Engineering Online at www.aidic.it/cet Guest Editors: Angelo Chianese, Luca Di Palma, Elisabetta Petrucci, Marco Stoller Copyright © 2016, AIDIC Servizi S.r.l., I SBN978-88-95608-38-9; I SSN 2283-9216 Synthesis of Macrocycle (MC) – Mimics the Properties of Natural Siderophores and Preparation the Nanostructures on the Basis of MC and Magnetite Nanoparticles Ulviyya Hasanova a* , Mahammadali Ramazanov a , Abel Maharramov a , Zarema Gakhramanova b , Sarvinaz Hajiyeva, Qoncha Eyvazova a , Leyla Vezirova b , Flora Hajiyeva a , Matanat Hasanova b , Narmina Guliyeva a a Baku State University, Z.Khalilov 23 Str., Baku, Azerbaijan b Azerbaijan State Oil Academy, RI Geotechnological properties of gas oil and chemistry, ulviyya3@rambler.ru In this work we report of the synthesis of hydroxyl containing azacrown macrocycle (MC) that is able to mimic the properties of natural siderophores. On the basis of synthesized MC were prepared the supramolecular ensembles with magnetite nanoparticles, loaded by cephalosporin antibiotics. The synthesized MC was investigated by NMR, mass-, FTIR spectroscopy methods. The morphology of prepared nano-ensembles was analyzed by scanning electron microscopy SEM, FTIR, X-ray diffraction XRD analysis methods. The quantitative analysis of nanostructures was determined by atom absorbance spectroscopy (AAS) as well as on the basis of Lambert-Beer law by UV spectroscopy method. Prepared nanostructures were tested on gram- negative Escherichia Coli and gram-positive Staphylococcus aureus, having multi drug resistance properties. Has been found that the nanostructures significantly increase the antimicrobial effect of cephalsporins and decrease their MIC. Introduction Today's huge problem, faced to clinicians, is the problem of antibiotic resistance. Synthesis and application of new drugs, for which the microorganisms have not yet worked out the mechanism of resistance, is extremely long and laborious process that requires significant investment. As the samples of resistance can be provided the fact, revealed by Brin et al. (2005), that the quantity of the ESBLs producing strains of the Escherichia Coli, causing nosocomial and community-acquired infections, is increasing rapidly day by day. In turn, according to the research of Adamuet et al (2010), Staphylococcus Aureus also show the rise of the number of resistant strains to antibiotics, especially to β-lactames, particularly in developing countries. As a result, there is an urgent requirement of new modified drugs that are effective against antibiotic-resistant bacteria. The one of the possible ways is nanostructuring of already used drugs that will enhance and improve their activity. In particular, as it was mentioned by Dorniani et al. (2013), attention is paid to the application of magnetite nanoparticles in targeted drug delivery, not only due to their biocompatibility, but also because of the possible targeting of these nanoparticles, loaded with drug, directly to the infected sites and organs of organism by means of the applied external magnetic field. And, in case with nanoantibiotics, as it was mentioned by Grumezescu et al., magnetite nanoparticles can reduce the toxicity and side effects of antimicrobial agents. It is known that the development and functioning of most pathogenic microorganisms require a great concentration of iron ions, and as a result they have developed a special system of binding and transport of iron ions that are necessary for the functioning of their life cycle. This system, as described by Miethke and Marahiel (2007), is based on a shuttle mechanism that uses small-molecule compounds, called siderophores, that selectively bind to the iron ions. Many scientists, including Kline et al. (2000), point that antibiotics, coupled with siderophore-mimic compounds, can easily penetrate through the membrane of gram-negative bacteria. DOI: 10.3303/CET1647019 Please cite this article as: Hasanova U., Ramazanov M., Maharramov A., Gakhramanova Z., Hajiyeva S., Eyvazova Q., Vezirova L., Hajiyeva F., Hasanova M., Guliyeva N., 2016, Synthesis of macrocycles (mc) – mimics the properties of natural siderophores and preparation the nanostructures on the basis of mc and magnetite nanoparticles., Chemical Engineering Transactions, 47, 109-114 DOI: 10.3303/CET1647019 109