~ 1544 ~ International Journal of Chemical Studies 2019; 7(4): 1544-1546 P-ISSN: 2349–8528 E-ISSN: 2321–4902 IJCS 2019; 7(4): 1544-1546 © 2019 IJCS Received: 01-05-2019 Accepted: 05-06-2019 Nagaratna N Koladar Department of Plant Pathology, College of Agriculture Junagadh Agricultural University, Junagadh, Gujarat, India JR Talaviya Department of Plant Pathology, College of Agriculture Junagadh Agricultural University, Junagadh, Gujarat, India SV Lathiya Department of Plant Pathology, College of Agriculture Junagadh Agricultural University, Junagadh, Gujarat, India IB Kapadiya Department of Plant Pathology, College of Agriculture Junagadh Agricultural University, Junagadh, Gujarat, India Correspondence Nagaratna N Koladar Department of Plant Pathology, College of Agriculture Junagadh Agricultural University, Junagadh, Gujarat, India In vitro evaluation of fungicide combination on turmeric anthracnose [ Colletotrichum capsici (Syd) butler and Bisby] Nagaratna N Koladar, JR Talaviya, SV Lathiya and IB Kapadiya Abstract Turmeric (Curcuma longa L.) is one of the most important spice crop cultivated in India. India is considered as the largest producer, consumer and exporter of turmeric in the globe. Turmeric is affected by anthracnose, so for its management combinations of fungicides evaluated under in vitro condition, the highest per cent inhibition was obtained by Cymoxanil + Mancozeb followed by Metiram + Pyraclostrobin and Carbendazim + Mancozeb in inhibiting the growth C. capsici at all the four different concentrations tested viz. 100, 250, 500 and 1000 ppm. Keywords: Evaluation of combinations of fungicides on turmeric anthracnose [Colletotrichum capsici (Syd.) butler and Bisby] Introduction Turmeric (Curcuma longa L.) is one of the most important spice crop cultivated in India. The crop yield is affected by several biotic and abiotic factors, among them, anthracnose of turmeric caused by Colletotrichum capsici was found increasing and occurring regularly every year. It has become as major constraint in successful cultivation of turmeric in Gujarat. Leaf spot disease of turmeric caused by C. capsici was reported for the first time from Coimbatore district of Madras by Mc Rae in 1917 [3] . Later, it was reported from turmeric growing regions like Cuddapah, Kurnool, Guntur, Krishna and Godavari districts of Andhra Pradesh and Coimbatore of Madras State (Ramakrishnan, 1954) [6] . Disease is soil-borne noticed on the leaves from July to October. In Gujarat, leaf spot of turmeric caused by C. gloeosporioides was first time reported by Patel et al. (2005) [5] . Leaf spot is the most important disease of turmeric resulting in losses of 25.83-62.12 per cent fresh weight and 42.10-62.10 per cent dry weight of rhizomes (Nair and Ramakrishnan, 1973) [4] . It causes extensive spotting of leaves. The leaves may eventually dry and thus adversely affect the formation of rhizomes. The incidence of turmeric leaf spot caused by C. capsici reported 50 per cent yield loss (Ramakrishnan, 1954) [6] . Hence, different combinations of fungicides tested against Colletotrichum capsici. Materials and Methods Different combinations of fungicides (Table 1) were tested for their effect on mycelium growth of C. capsici using poisoned food technique (Sinclair and Dhingra, 1985) [8] at four concentrations. The technique involves cultivation of test organism on a medium containing the test chemical. In experiment PDA was used as a basal medium. The calculated quantities of fungicides were thoroughly mixed in the molten almost cool PDA medium before pouring into Petri plates aseptically, so as to get desired concentration of each fungicide separately. 20 ml of fungicide amended medium was poured in each 90 mm sterilized Petri plates and allowed to solidify. The plates were aseptically inoculated with 5 mm disc cut from the periphery of 12 days of old actively growing cultures of C. capsici. Controls without fungicides amended were maintained for comparison. The experiments were conducted in completely randomized design with three replication of each treatment and the inoculated plates were incubated at 28±2°C. The colony diameter was measured after 7 days when the control plates were full of fungal growth. Per cent inhibition of growth of mycelium for each treatment was calculated by using the formula given by Vincent (1947) [9] .