Research Article Characterizationofthe Omija (Schisandra chinensis)Extractand Its Effects on the Bovine Sperm Vitality and Oxidative Profile during In Vitro Storage EvaTvrd´ a , 1 Jaroslav Michalko, 2,3 J´ ulius ´ Arvay, 4 Nenad L. Vukovic, 5 Eva Ivaniˇ sov´ a, 6 Michal ˇ Duraˇ cka, 1 Ildik´ o Matuˇ s´ ıkov´ a, 7 and Miroslava Kaˇ c´ aniov´ a 8,9 1 Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia 2 BioFood Center, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia 3 Detached Branch of the Institute of Forest Ecology-Arbor´ etumMlyˇ nany, Slovak Academy of Sciences, Vieska Nad ˇ Zitavou 178, 951 52 Slepˇ cany, Slovakia 4 DepartmentofChemistry,FacultyofBiotechnologyandFoodSciences,SlovakUniversityofAgricultureinNitra,Tr.A.Hlinku2, 949 76 Nitra, Slovakia 5 Department of Chemistry, Faculty of Science, University of Kragujevac, 34000 Kragujevac, Serbia 6 Department of Technology and Quality of Plant Products, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, Slovakia 7 Department of Ecochemistry and Radioecology, Faculty of Natural Sciences, University of SS. Cyril and Methodius in Trnava, N´ am. J. Herdu 2, 917 01 Trnava, Slovakia 8 Department of Fruit Sciences, Viticulture and Enology, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, Slovakia 9 Department of Bioenergy, Food Technology and Microbiology, Institute of Food Technology and Nutrition, University of Rzeszow, Zelwerowicza St. 4, 35601 Rzeszow, Poland CorrespondenceshouldbeaddressedtoEvaTvrd´ a; evina.tvrda@gmail.com Received 8 May 2020; Accepted 14 September 2020; Published 22 September 2020 Academic Editor: Filippo Fratini Copyright©2020EvaTvrd´ a et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Schisandra chinensis isawoodyvinenativetoChina,Korea,andRussia,whichhasbeenusedasatraditionalherbalremedy to treat male infertility. As very little information is available concerning its effects on ejaculated spermatozoa, the aim of thisstudywastoinvestigatethechemical,antioxidant,andantibacterialpropertiesofthe S. chinensis berry(Omija)extract followedbyanassessmentofits invitro effectsonbovinespermfunctionandoxidativebalance.Phytochemicalcomponents ofthe Omija extractweredeterminedbyhighperformanceliquidchromatography.econtentofpolyphenols,flavonoids, and carotenoids was assessed by spectrophotometric protocols. Antioxidant characteristics of the Omija extract were determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and molybdenum-reducing antioxidant power (MRAP) assays. e disc diffusion method and determination of the minimal inhibitory concentration were applied to study the anti- bacterialpropertiesof Schisandra.irtysemensampleswereexposedtodifferentconcentrationsof Omija (1,5,10,25,50, and 75 µg/mL) for 0, 2, and 24h. Sperm motility, mitochondrial activity, and superoxide and reactive oxygen species production, as well as total antioxidant capacity and oxidative damage to proteins and lipids were determined. Our data reveal that the Omija extract, particularly at a concentration range within 5–50 µg/mL, exhibited dose-dependent motion- promotingandmetabolism-enhancingproperties,accompaniedbysignificantantioxidanteffects.Wemayconcludethatthe biomolecules present in the Omija extract such as schisandrins and phenolic molecules offer protection to critical sperm structuresagainstoxidativeinsultsand/orpossiblebacterialcontamination,leadingtoahigherpreservationofmammalian sperm viability and functional activity. Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2020, Article ID 7123780, 15 pages https://doi.org/10.1155/2020/7123780