Research J. Pharm. and Tech. 12(12): December 2019 6119 ISSN 0974-3618 (Print) www.rjptonline.org 0974-360X (Online) RESEARCH ARTICLE Comparing The levels of trace Elements in females with Diabetes Mellitus Seenaa kadhum Ali 1 , Zainab Abbas jwad 2 , Hayder Noori Raheem 3 , Hayder yaseen Noor 4 , Ayad Kadhim Ali 5 1 Chemistry Deptartment, Faculty of Education for women, Kufa University, Najaf city, Iraq 2 Chemistry Deptartment, College of Science, University of Babylon, Hilla city, Iraq 3 Chemistry Deptartment, Faculty of Pharmacy Pharmaceutical, Kufa University, Najaf city, Iraq 4 Chemistry Deptartment, Faculty of Pharmacy Pharmaceutical, Kufa University, Najaf city, Iraq 5 Soil Science and water Dept, Faculty of Agriculture, Kufa University, Najaf city, Najaf Governorate, Iraq *Corresponding Author E-mail: seenaa.alhusseini@uokufa.edu.iq ABSTRACT: Cumulative investigations have depicted that metabolic rate of some trace elements has altered in diabetes. This study is designed to illustrate the consequence of some trace element copper (Cu), iron (Fe), Cobalt (Co), zinc (Zn) and Zn/Cu ratio) in diabetic female patients with mellitus type 2 (n=60), type 1 (n=69) as compared to nondiabetic control subjects (n=35). The element levels have recorded by an atomic absorption spectrophotometer. The consequences of this paper have depicted that the mean magnitudes of Zn, Cu, in addition to Fe have significantly lessened in testers of diabetic patients than control subjects (p < 0.006, p < 0.000, p < 0.000) correspondingly, but no significances in serum cobalt. Serum Cu, Fe levels have been significant and lower in samples of type 1 diabetic patients than type 2 diabetic patients (p < 0.001, p < 0.005). KEYWORDS: Comparing, Trace Elements, Females, Diabetes Mellitus. INTRODUCTION: Diabetes mellitus is cluster of metabolic syndromes that characteristics in terms of raised blood glucose status (hyperglycemia) causing from imperfections in insulin action, insulin secretion, or together. Chronic hyperglycemia is linked with dysfunction, longstanding damage, and failure of diverse organs, mainly the kidneys, eyes, blood vessels, heart, and nerves (1) . Symptoms of noticeable hyperglycemia consists of blurred vision ,weight loss, polyuria, sometimes with polydipsia and polyphagia (2) . Trace elements have testified to be vital cofactors of anti-inflammation and antioxidant system (3, 4) . The trace elements should be presented at appropriate levels in the body. Zn stands for an indispensable trace element in the body and it is needful for the organisms growth. It stands for secondly high abundant trace element in the humanoid body following Fe Zinc can be found in all body tissues and fluids. Received on 11.06.2019 Modified on 19.07.2019 Accepted on 13.08.2019 © RJPT All right reserved Research J. Pharm. and Tech. 2019; 12(12): 6119-6123. DOI: 10.5958/0974-360X.2019.01063.1 Zinc is required for over 300 enzymes. Recommended Dietary Allowance (RDA) of Zn is 11 mg meant for males and 8 mg for females. Doses greater than 25 mg can instigate anemia and Cu deficiency (5) . It also regulates enzymatic action and the stability of proteins as an activator or as an inhibitor ion (6) . Zinc has the possibliy for modulating cellular signal reception, 2 nd messenger metabolic rate, and protein kinase as well as protein phosphatase actions (7) . Cu concentration in the humanoid body is a significant indicator of health, As a cofactor in Cu/Zn-SODs. Deviations in plasma levels of Cu besides the imbalanced ratio of Cu/Zn have been verified to be indicators of infection, vascular problems, and other diseases (8) . Cu is an essential trace element, Cytochrome oxidase lysyl oxidase,transcription factors and Zn-Cu SOD are Critical proteins all require Cu as an essential element (9) Copper (Cu) deficiency was assossation with impaired glucose tolerance in previously study (10) . In the human body, Iron stands for highly abundant nutritional trace element. It plays a critical role in