e278 13th International Congress on Infectious Diseases Abstracts, Poster Presentations CA-INT-L and CA-INT-R sequences that span the transposable intron in 25SrDNA. Results/Discussion: Fluconazole susceptibility (MIC ≤8 g/ml) was 52/92(56.5%) while Fluconazole suscep- tible dose dependant (S-DD) (MIC 16—32 g/ml) was 28/92(30.5%). There were 12/92(13.0%) isolates with MIC ≥ 64 g/ml to fluconazole. The MIC90 and MIC50 of the isolates were 16 and 8 g/ml, respectively. Most of the isolates were susceptible to amphotericin B with > 90% of the isolates with MIC of ≤ 0.25 g/ml and only two isolates with MIC ≥1 g/ml. Candida albicans genotype A, B and C were identified with genotype A being the most (60%) predominant. Due to life long fluconazole maintenance therapy in HIV/AIDS, there is need for constant surveillance for emerging azoles resistance and strengthening technical and infrastructural capabilities for diagnosis and research in HIV/AIDS associated opportunistic infections. doi:10.1016/j.ijid.2008.05.746 45.005 Fungemia in Non-HIV-infected Patients: A 5-Year Review S. Anunnatsiri ∗ , P. Chetchotisakd, P. Mootsikapun Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand Objectives: To investigate the incidence, risk factors, causative fungi and outcomes of fungemia in adult, non-HIV- infected patients Design: We studied 147 episodes of fungemia due to Can- dida spp., Torulopsis spp., and Trichosporon spp. in adult patients admitted to a university hospital in Northeast Thai- land between 1999 and 2003. Results: The overall incidence of fungemia was 14.1 per 10 000 hospital admissions. Candida was the most common isolation (126 episodes; 85.7%) with non-albicans candida accounting for 60.5%. The major non-albicans candida iso- lations were C. parapsilosis and C. tropicalis. Fungemia caused by Trichosporon and Torulopsis accounted for 14.3% of the cases but their clinical features could not be dis- tinguished from fungemia due to Candida. The overall in-hospital mortality rate was 56.1%. The independent fac- tors related to the mortality outcome were high APACHE II score (OR 1.099 per 1-point increments; 95%CI 1.004—1.204) and assisted ventilation (OR 4.03; 95%CI 1.21—13.41). Conclusions: Candidemia, especially non-albicans can- dida, was an important nosocomial infection in this tertiary hospital in Northeast Thailand. The mortality rate was high, particularly in critically-ill patients. Rapid diagnosis and early treatment are therefore important challenges to improving clinical outcomes. doi:10.1016/j.ijid.2008.05.747 45.006 Rhino-Facial Entomophthoromycosis Due to Conidiobolus Coronatus R. Tavares Egas Moniz Hospital, Lisbon, Portugal Conidiobolomycosis or rhinoentomophthoromycosis is caused by Zygomycetes of the order Entomophthorales. Conidiobolus coronatus infection is an unusual fungal infec- tion. Conidiobolus is found worldwide in soils and plants debris, but occurs at higher concentrations in warm coun- tries, mainly in Africa and India, during the rainy season. This fungus can infect immunocompetent mammals, includ- ing humans. The mode of transmission is probably inhalation of fungal spores, which implant in nasal mucosa and cause an orofacial granulomatosis. The differential diagnosis of facial swelling should always include testing for fungal infections. This clinical case reports on a 29-year-old male patient, pre- viously healthy, coming from Guinea Bissau to Portugal, to investigate facial swelling, with the diagnosis hypothesis of brain cancer, which was later excluded. Two years previ- ously, the patient had presented facial swelling and solution in palate. First, he was treated with steroids but showed no improvement. Diagnosis was severely delayed, because the first biopsies were inconclusive: no agent was detected except Mycobacterium tuberculosis. The patient started treatment, however facial swelling worsened, with occlu- sion of the eyes and mouth. More biopsies were performed and only surgical management allowed us to obtain sam- ples of nasal tissues, in which Conidiobolus coronatus was found. Following the diagnosis of orofacial conidiobolomyco- sis, treatment with fluconazole, saturated solution of iodide and trimetropim sulphametoxazol, was started with clini- cal improvement. With the discussion of this clinical case, we aim to alert practitioners to the existence of this kind of unusual fungal infection in immunocompetent patients and for the difficulty in getting the right samples to permit diagnosis. doi:10.1016/j.ijid.2008.05.748 45.007 Monitoring on Antifungal Resistance from Clinical Candida Species by E-Test P. Badiee ∗ , A. Alborzi, E. Shakiba, A. Joponi Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran (Islamic Repub- lic of) Background: Although there has been a significant increase in the number of reported infections caused by yeasts of the genus Candida(C), Candida albicans is the most frequent isolated Candida species. The aim of this study was molecular identification of C. dubliniensis from C. albicans suspension yeasts, and evaluation the in vitro activity of flu- conazole, amphotericin B, ketoconazole, itraconazole, and voriconazole against the isolates. Methods: From October 2003 to March 2007, the clin- ical samples, which were sent to Clinical Microbiological Research Center, Nemazi Hospital, Shiraz, Iran were ana-