Oral – [A-10-175-1] The improvement of antioxidant status in aging mice sperm following sodium selenite treatment Shabnam Mohammadi a , Majid Ghayour Mobarhan a , Seyed Javad Movla b a Mashhad University of Medical Sciences, Mashhad, Iran b Tehran, Iran E-mail addresses: mohammadish881@mums.ac.ir (S. Mohammadi), ghayourm@mums.ac.ir (M. Ghayour Mobarhan), movla@yahoo.com (S.J. Movla) Introduction: Selenium as an antioxidant plays a crucial role in normal function of testis and spermatogenesis. It can reduce formation of free oxidative radicals and as a result it is expected to increase male fertility. This study aimed to investigate the effects of selenium supplementation on antioxidant status of aging and adult mice sperm. Materials and methods: Fifteen male mice with 10–12 months aged and fifteen male mice with 2–3 months aged were randomly divided into three groups: control, sham and experimental groups. The control group did not receive any treatment. The sham group mice were injected with selenium solvent with the same volume as the experimental group. Experimental groups of male mice were injected intraperitoneally daily with a dose of 0.2 mg/kg sodium selenite for 5 weeks. Mice were sacrificed by cervical dislocation on day 42 after selenium treatment and after finding sperm, total antioxidant capacity of sperm was measured by FRAP assay. The absorbance of TPTZ-Fe 2+ was read in 593 nm by a spectrophot- ometer. The data were analyzed using SPSS software and Kruskal Wallis and were assumed significant when P<0.05. Results: Our results showed that the mean values of total antioxidant capacity increased both in aging and adult mice (P <0.05). Also, mean value of total antioxidant capacity in adult control group was 742.26±1.06 while this value was 672.061±0.78 for the aging control group (P < 0.05). Conclusion: Selenium supplementation in aging mice can improve total antioxidant capacity of sperm and antioxidant therapy could be considered to improve sperm of aging men. Keywords: Sodium selenite, Testis, Mice, Antioxidant, Selenium doi:10.1016/j.clinbiochem.2011.08.1040 Oral – [A-10-509-1] Strontium-substituted bioactive glass coatings for bone tissue engineering Nasrin Lotfibakhshaiesh a , Eileen Gentleman a , Robert Hill b , Molly Stevens a a Imperial College London, SW7 2AZ, London, UK b Qeen Mary University, Mile End Road, E1 4NS, London, UK E-mail address: nlotfiba@imperial.ac.uk (N. Lotfibakhshaiesh) Introduction: Metallic prosthetic implants are widely used to treat joint and skeletal injuries and disease. However, some implants can fail because the metal alloys used do not bond with bone. Bioactive glass (BG) coatings may offer a solution to this problem as they form a strong bond with living tissue and have the added benefit that their dissolution ions stimulate cell activity1. Strontium (Sr) is known as a trace element in the body and can be used as a drug to treat and prevent osteoporosis which stimulate the formation of new bone and prevent osteoclast-mediated resorbtion2. Sr can be substituted for calcium in BGs creating a material that may combine the bone remodelling benefits of Sr ions with the well-established bone stimulatory action of bioactive glass. The aim of this study is to develop Sr containing BG coatings on metallic surfaces to assess their effectiveness in bonding to an implant as an effective biomaterial choice for a range of bone regeneration therapies. Materials and methods: BGs in the system: SiO2-MgO-Na2O- K2O-ZnO-P2O5-CaO with various concentration of Sr were prepared. Bioactivity was tested by simulated body fluid and analysed by XRD. Culture media containing ions from glasses were prepared by incubating of glass powder in RPMI. The human osteosarcoma cell line was cultured and cell metabolic activity measured. Glasses were coated on the surface of Ti coupons. Saos-2 cells were seeded on BG coatings; viability was assessed with a LIVE/DEAD stain. BG coatings cultures were fixed, gold coated and viewed using SEM. Results: Glass dissolution leads to a rise of all the ion concentra- tions in solution. MTT activity increased in all samples with time in culture. Saos-2 cultured treated with Sr-substituted BGs had higher MTT activities than controls (p <0.01). Live/Dead staining showed that cells were alive on all coating materials. SEM imaging demonstrated that the BG coating encouraged cell attachment. Conclusion: Substituting strontium for calcium increases the dissolution of the glass releasing in more ions in solution. These ions may tend to enhance cells’ metabolic activity, which may lead to an increase in mineralization. As BGs are a well-studied biomaterial system capable of controlled ion release and are deliverable as coatings or scaffolds substitution of strontium for calcium in bioactive glasses may be an effective strategy for creating materials for bone repair/regeneration therapies. Keywords: Strontium, Bioactive glass coatings, Bone doi:10.1016/j.clinbiochem.2011.08.1041 Oral – [A-10-1097-1] Immunological association between antioxidant trace elements and susceptibility to allergic rhinitis Hadi Hassannia, Saeid Abedian, Javad Ghaffari, Elham Biranvand, Fereshteh Jeivad, Ghorban Gohari Mazandaran University of Medical Sciences, Mazandaran, Iran E-mail address: Hadi3977@yahoo.com (H. Hassannia) Introduction: Allergic rhinitis (AR) is a common chronic inflammatory disease of nasal airways and characterized by Th2- deviated immune response. Trace elements such as Zinc and Copper (Zn and Cu) are important immune modulators and essential cofactors of the antioxidant enzymes. This study is designed to evaluate the role of Zn and Cu concentrations in allergic rhinitis patients. Materials and methods: In this case–control study, 318 blood samples were collected from 155 AR patients and 163 allergy-free controls which matched according to age, gender and living area. Serum Zn and Cu concentrations were determined using colorimetric test. Also, the relationship between these elements and total serum IgE and IgA levels and peripheral blood eosinophil counts were evaluated in this population; serum IgE and IgA were measured by ELISA and nephlometric method respectively and eosinophil counts were determined based on the eosinophil numbers per total cell numbers. Statistical significance was defined as p<0.05 (using SPSS 18). Results: Serum Zn level in patient group was significantly lower than healthy group (p=0.001). However, there was no significant difference in serum Cu level between two groups (p>0.05). Also, statistically, there was significant difference in eosinophilia percentage and serum IgE and amp; IgA levels among allergic rhinitis and control groups (p < 0.05). Conclusion: Our findings indicated that Zn deficiency might be associated with the susceptibility and pathogenesis of allergic rhinitis. Abstracts S36