Volume 65, Issue 1, 2021 Journal of Scientific Research Institute of Science, Banaras Hindu University, Varanasi, India. 186 DOI: 10.37398/JSR.2021.650124 Abstract: Tumor vascularity and lymphangiogenesis are two established prognostic parameters however detecting a proxy marker could help in prognosticating patients in an early stage. We tried to investigate color doppler and Lymphatic vessel immunohistochemical staining in breast cancer patients and correlate it with established prognostic markers in breast cancer. 30 patients were included in the study with the mean age of 44.40±10.37 years (range 30-65 years). In color doppler, high RI value significantly correlated with T3 tumor size, number of lymph nodes (>10), grade (grade III), hormonal status, LMVD (high) and LVI D2- 40 positivity with a p-value of 0.0004, 0.001, 0.007, 0.006, 0.008 and 0.008 respectively. On correlation with PI, number of lymph nodes (>10), grade (grade III), hormonal status and LVI D2-40 positivity were significantly correlated with high PI value (p=0.044, p=0.012, p=0.013 and p=0.046 respectively). In Lymphatic vessel immunohistochemical staining high LMVD value significantly correlated with tumor size, number of lymph nodes, tumor grade and hormonal status (p=0.002, p=0.001, p=0.0006 and p=0.015) while LVI significantly correlated with tumor size, number of lymph nodes and hormonal status prognostic factors (p=0.008, p=0.013 and p=0.046 respectively). Index Terms: Breast cancer, Color Doppler, Immunohistochemical staining, Lymphangiogenesis, Tumor vascularity. I. INTRODUCTION Breast cancer (BC) is the third most common tumor in world and represents 9% of global cancer burden (Lopez-Knowles et al, 2004). In India it is the second commonest cancer amongst * Corresponding Author women next to cancer cervix with a rising incidence in young premenopausal women (Raina et al, 2005). Over the past few decades, BC management has undergone significant changes, characterized by less aggressive and rationalized approaches in diagnosis and treatment. Current management of primary BC involves the need for assessment of various predictors and prognostic markers. The prognostic markers are clinical or biological parameters associated with disease free or overall survival. In an attempt to predict the fate of patient, various prognostic factors have been identified. The functional assessment of tumor angiogenesis and neovascularization has now become possible with the use of color Doppler. Osanai et al (2003) and Kumar et al (2007, 2010) in their study confirmed that preoperative color Doppler ultrasonography is useful for the assessment of intratumoural blood flow analysis, correlates well with histological grade and degree of malignancy of the BC. BC have been found to be associated with higher values of doppler parameters like resistivity index (RI), pulsatility index (PI) and maximum flow velocity (Vmax) (Chao et al, 1999). Estimation of lymphovascular invasion and tumor lymphangiogenesis assessment are current emerging prognostic indicators (Hasebe et al, 2004 & Schoppmann et al, 2004). Lymph node (LN) status is the most important independent clinical prognostic factor for patients with BC (Fischer et al, 1995). The presence and the extent of axillary LN metastases reflect the probability that the cancerous process has spread through the body and both are strongly correlated with the development of distant metastases and with shortened disease- Prognostic Correlation of Color Doppler Ultrasonography and Lymphangiogenesis in Breast Carcinoma Vivek Srivastava * , Vikash Singh, Pratik K Jha, Mumtaz A Ansari, and Anand Kumar Department of General Surgery, Institute of Medical Sciences, BHU, Varanasi. vivekims97@gmail.com*, vikassingh9397@gmail.com, pratik.kumar17@gmail.com, mumtazbhu@gmail.com, profanandkumar52@gmail.com