Research Article Toxicity Mitigation of Textile Dye Reactive Blue 4 by Hairy Roots of Helianthus annuus and Testing Its Effect in In Vivo Model Systems Kanchanlata Tungare , 1 Rinkey Shahu , 2 Vyankatesh Zambare , 3 Payal Agarwal , 2 Renitta Jobby , 2,4 Nazima Nisar, 5 Nadiyah M. Alabdallah , 6 Fatimah A. Al-Saeed , 7 Parul Johri, 8 Sachidanand Singh, 9 Mohd Saeed , 10 and Pamela Jha 11 1 School of Biotechnology and Bioinformatics, D. Y. Patil Deemed to be University, Plot No. 50, Sector 15, CBD Belapur, 400614 Navi Mumbai, Maharashtra, India 2 Amity Institute of Biotechnology, Amity University Maharashtra, Mumbai Pune Expressway, Bhatan, Post Somatane, Panvel, Mumbai, Maharashtra 410206, India 3 Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park PA 16802, USA 4 Amity Centre of Excellence in Astrobiology, Amity University, Maharashtra-Pune Expressway, Bhatan, Panvel, Mumbai, Maharashtra 410206, India 5 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia 6 Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, 31441 Dammam, Saudi Arabia 7 Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia 8 Department of Biotechnology, Dr. Ambedkar Institute of Technology for Handicapped, Kanpur 208001, India 9 Department of Biotechnology, Smt. S. S. Patel Nootan Science & Commerce College, Sankalchand Patel University, Visnagar, 384315 Gujarat, India 10 Department of Biology, College of Sciences, University of Hail, Hail, Saudi Arabia 11 Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS Deemed to be University, Vile Parle (West), Mumbai 400056, India Correspondence should be addressed to Mohd Saeed; mo.saeed@uoh.edu.sa and Pamela Jha; pamelajha@gmail.com Received 16 May 2022; Revised 24 June 2022; Accepted 25 June 2022; Published 25 July 2022 Academic Editor: Abdelaaty Shahat Copyright © 2022 Kanchanlata Tungare et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. An anthraquinone textile dye, Reactive Blue 4 (RB4), poses environmental health hazards. In this study, remediation of RB4 (30- 110 ppm) was carried out by hairy roots (HRs). UV-visible spectroscopy and FTIR analysis showed that the dye undergoes decolourization followed by degradation. In addition, toxicity and safety analyses of the bioremediated dye were performed on Allium cepa and zebrafish embryos, which revealed lesser toxicity of the bioremediated dye as compared to untreated dye. For Allium cepa, the highest concentration, i.e., 110 ppm of the treated dye, showed less chromosomal aberrations with a mitotic index of 8:5±0:5, closer to control. Two-fold decrease in mortality of zebrafish embryos was observed at the highest treated dye concentration indicating toxicity mitigation. A higher level of lipid peroxidation (LPO) was recorded in the zebrafish embryo when exposed to untreated dye, suggesting a possible role of oxidative stress-inducing mortality of embryos. Further, the level of LPO was significantly normalized along with the other antioxidant enzymes in embryos after dye bioremediation. At lower concentrations, mitigated samples displayed similar antioxidant activity comparable to control underlining the fact that the dye at lesser concentration can be more easily degraded than the dye at higher concentration. Hindawi BioMed Research International Volume 2022, Article ID 1958939, 13 pages https://doi.org/10.1155/2022/1958939