2337 EurAsian Journal of BioSciences Eurasia J Biosci 13, 2337-2342 (2019) Wound healing potency of Terminalia catappa in mice ( Mus musculus) Rudy Agung Nugroho 1* , Retno Aryani 1 , Hetty Manurung 2 , Rudianto 1 , Dinda Prameswari 1 1 Laboratorium Fisiologi, Perkembangan dan Molekuler Hewan, Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman, Samarinda, Kalimantan Timur, INDONESIA 2 Laboratorium Fisiologi Tumbuhan, Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman, Samarinda, Kalimantan Timur, INDONESIA *Corresponding author: rudyagung.nugroho@fmipa.unmul.ac.id Abstract Leaves of Terminalia catappa and its phytoconstituents are well known as a natural promising medicine especially for wound healing agents. The purpose of the current research was to evaluate the wound healing potency of ethanolic and water extract of T. catappa either green or brown leaves in mice (Mus musculus) model. A group of mice were used and randomly divided into control, vaseline, povide iodine groups and treatment groups consisting ethanolic extract either green (GLE) or brown leaves (BLE), and water extract green (GLW) and brown (BLW) leaves of T. catappa as ointment. All mice were prepared for excision and treated with simple ointment once a day until the wound was enclosure. The percentage of wound enclosure was recorded every three days, while total DNA and hydroxyproline content were determined at day 12. Qualitative phytochemical assay was performed to determine the phytoconstituent in the leaves extract. The results found that mice treated with GLE and GLW had better wound healing at the day12, confirming that the wound healing activity was found to be better than control and Povidone-iodine group. Furthermore, hydroxyproline content were also found to be higher in all treated groups than control group. Present finding suggested that T. catappa leaves extract which contains important phytochemicals shows a good wound healing activity and can be used as alternative medicine for wound healing. Keywords: mice, phytochemicals, Terminalia catappa, wound healing Nugroho RA, Aryani R, Manurung H, Rudianto, Prameswari D (2019) Wound healing potency of Terminalia catappa in mice (Mus musculus). Eurasia J Biosci 13: 2337-2342. © 2019 Nugroho et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License. INTRODUCTION The Indian almond tree or known as Terminalia catappa is a native to Southeast Asia and widely distributed in both tropical and sub-tropical regions. The T. catappa is an attractive, long-lived tree well suited to ornamental and amenity plantings. The leaves of T. catappa are useful as a folk medicine such as antidiabetic, analgesic, antiulcer, hyper glycaemia, and liver-related diseases (Anand et al. 2015, Behl and Kotwani 2017, Pinheiro Silva et al. 2015). Previously reported also obtained that the leaf of T. catappa has an antibacterial properties (Allyn et al. 2018, Nugroho et al. 2017) and it contains benefit phytochemicals such as saponin, triterpenoid, quinone, phenolic, tannin, and flavonoid (Nugroho et al. 2016), that may be useful for wound healing. Wound healing is a natural repairing process of the damage tissue that consists of four biochemical events: blood coagulation, inflammation which is immediately begins after injury, cell proliferation, lesion contraction and remodeling (Ghuman et al. 2019, Hermes et al. 2013). In the proliferation event, a forming of granulation tissue is built by fibroblast and the angiogenesis process (Dwivedi et al. 2017). According to Lodhi and Singhai (2013), a successful of wound healing process depends on the several factors such as blood cells and parenchymal cells component in a time frame, extracellular matrix molecules, soluble and biochemical mediators. Further, determining the DNA and hydroxyproline content at wound healing tissue is a well-established procedure for monitoring normal cell proliferation (Nordin et al. 2018).The enhance in the DNA and hydroxyproline content is a result of increased cell division (Ahmad et al. 2017). Moreover, the increase of DNA content can be stimulated by Platelet-derived growth factor (PDGF) which also triggered chemotaxis of fibroblasts and production of collagen, glycosaminoglycan, and collagenase (Lynch et al. 1987). Meanwhile, previous study also revealed that there was a high content of hydroxyproline due to the Received: June 2019 Accepted: November 2019 Printed: December 2019