Indian Journal of Natural Sciences www.tnsroindia.org.in ©IJONS Vol.8 / Issue 47 / April 2018 International Bimonthly ISSN: 0976 – 0997 13604 The Role of Pumpkin Seed Oil in Healing of Wounds in Diabetic Mice Nabaa Fadil Abbas 1 * and Falah Muosa Kadhim Alrekabi 2 1 MSc student, Department of Physiology ,Biochemistry and Pharmacology , College Of veterinary Medicine , University of Baghdad-Iraq 2 PhD ,MSc , Department of Physiology ,Biochemistry and Pharmacology , College Of veterinary Medicine ,University of Baghdad-Iraq Received: 11 Feb 2018 Revised: 24 Feb 2018 Accepted: 31 Mar 2018 * Address for correspondence Nabaa Fadil Abbas M.Sc Student, Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Baghdad,Iraq. E mail: nabaabass8@gmail.com This is an Open Access Journal /article distributed under the terms of the Creative Commons Attribution License (CC BY-NC-ND 3.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. All rights reserved. This study conducted to explore the role of pumpkin seed oil of Cucurbita pepo ,as alternative of traditional medicinal treatment for management wound healing in diabetic mice. Firstly ,the oil of Cucurbita Pepo (pumpkin seed oil) obtained by hot extraction method with n-hexane . Forty male Balb- c mice ,positively induced diabetes by single intra peritoneal dose of alloxan 150mg/kg BW, and surgical bilateral skin wounds performed on the back region ,after they were provide diabetic. The mice received daily insulin therapy 14 Iu/kg.bw along the period of the experiment . Clotting time ,platelet counts, blood glucose, insulin concentration ,white blood cells count, red blood cells count, differential white blood cells count ,pro thrombin time ,thrombin time and fibrinogen level ,were assessed after one day of incision and after complete wound healing in all experimental animals . The wounds were followed up through measuring their diameters, photographically and histologically (through obtaining skin biopsy after 4,6,8,10 day and so on of incision till healing) . The result showed, the extraction yield of C. pepo seed is 58% oil (pumpkin seed oil), and the phytochemical analysis of both pumpkin seed oil and powder proved presence of saponin ,flavonoid and steroid. There were no significant p<0.05 changes in clotting time ,platelet count ,red blood cells count and pro thrombin time between their values after one day of incision and after healing of all experimental groups . There were significant p<0.05 decrease in serum insulin concentration ,blood glucose level , white blood cells count ,lymphocytes count and thrombin time of groups treated with PSO and PSO plus MEBO when compared between their values after healing to those after one day of incision , but the fibrinogen level was significantly p<0.05 increase in the group treated with pumpkin seed oil only .The diameter of wounds of all experimental ABSTRACT RESEARCH ARTICLE