~ 253 ~ The Pharma Innovation Journal 2019; 8(1): 253-255 ISSN (E): 2277- 7695 ISSN (P): 2349-8242 NAAS Rating: 5.03 TPI 2019; 8(1): 253-255 © 2019 TPI www.thepharmajournal.com Received: 10-11-2018 Accepted: 12-12-2018 Abdul Mukit Barbhuiya Department of Pharmacology, Anurag Pharmacy College, Ananthagiri (V), Kodad (M), Nalgonda, Telangana, India P Hima Bindu Department of Pharmacology, Anurag Pharmacy College, Ananthagiri (V), Kodad (M), Nalgonda, Telangana, India D Preethi Department of Pharmacology, Anurag Pharmacy College, Ananthagiri (V), Kodad (M), Nalgonda, Telangana, India M Ramya Sri Department of Pharmacology, Anurag Pharmacy College, Ananthagiri (V), Kodad (M), Nalgonda, Telangana, India R Sai Sindhu Department of Pharmacology, Anurag Pharmacy College, Ananthagiri (V), Kodad (M), Nalgonda, Telangana, India Correspondence Abdul Mukit Barbhuiya Department of Pharmacology, Anurag Pharmacy College, Ananthagiri (V), Kodad (M), Nalgonda, Telangana, India Evaluation of In-Vitro thrombolytic activity of Manilkara zapota leaf extract Abdul Mukit Barbhuiya, P Hima Bindu, D Preethi, M Ramya Sri, R Sai Sindhu Abstract The present study was aimed to evaluate the in vitro thrombolytic activity of Manilkara zapota for the potential against thrombosis by dissolving the clot. The fresh leaves of Manilkara zapota were collected, grinded to obtain coarse powder then subjected to hydro ethanolic extraction by Soxhlet apparatus. Finally the extracts were air dried and then evaluated for thrombolytic activity according to Prasad S et al described method. The results of study were tabulated and analyzed for the thrombolytic activity and it is found that the hydro ethanolic extract of the plant possess moderate thrombolytic activity which may be due to the presence of several phytoconstituents in the plant extract. Keywords: Herbal medicines, Manilkara zapota, thrombolytic activity, extract. Introduction Herbal medicines are defined as a plant or plant part or an extract used for the treatment of several diseases [1] . In the recent trends the demand of herbal medicines is increasing exponentially. Because of public as well as medical establishments, studies leading to the scientific explanation of plant therapeutic capabilities are allowing this practice to gain increasing credibility and acceptance within the medical community [2] . Rapid changes in lifestyles of people on the globe enhance the occurrence of chronic non- communicable diseases including obesity, diabetes mellitus, cardiovascular disease (CVD), hypertension, stroke and some types of cancer which are significant causes of disability and pre mature death in developing and newly developing countries, placing additional burdens on already overtaxed national health budget [3] . The importance of dietary supplements derived from plants has accelerated in recent years because plants have remedy for various diseases. Pharmacologists, microbiologists, botanists, and natural-products chemists are engaged to develop a good herbal drug for treatment of various diseases. In fact, according to the World Health Organization, approximately 25% of modern drugs used in the United States have been derived from plants [4] . Many of the pharmaceuticals currently available to physicians have a long history of use as herbal remedies, including opium, aspirin, digitalis, and quinine. At least 7,000 medical compounds in the modern pharmacopoeia are derived from plants [8] . All plants produce chemical compounds as part of their normal metabolic activities, these phytochemicals are mainly responsible for the healing of the disease [5] . Manilkara zapota, commonly known as the sapodilla whose compounds extracted from the leaves showed anti-diabetic, antioxidant and hypocholesterolemic effects in rats [6] . Keeping this in view, the present study is aimed to evaluate the in vitro thrombolytic activity of Manilkara zapota for the potential against thrombosis by dissolving the clot. Materials and Methods Collection of plant part The fresh leaves of Manilkara zapota were collected from the botanical garden of Anurag Pharmacy College, Kodad. Drying, extraction and fractionation The fresh leaves of Manilkara zapota were cleaned and then it was grinded to obtain coarse powder of standard size suitable for extraction.