147 | Page THE ANALYTICAL STUDY OF ANTIGENICITY AND MHC BINDER OF THE CYTOCHROME C OXIDASE SUBUNIT I Sonu Mishra 1 , Virendra S. Gomase 2 , Karbhari V. Kale 3 1,2 Department of Biotechnology, Mewar University, Chittorgarh, (India) 3 Dept. of Computer Science and IT, Dr.Babasaheb AmbedkarMarathwada University, Aurangabad, MS (India) ABSTRACT In this study we will summarize the potency of Cytochrome c oxidase subunit I (mitochondrion )from Dracunculus medinensis with526 amino acids. Antigenic peptide of cytochrome c oxidase subunit I (mitochondrion) protein is most suitable for subunit vaccine development because with single epitope, the immune response can be generated in large population. In this investigation, we used PSSM and SVM algorithms for the prediction of MHC class I & II binding peptide, antigenicity, solvent accessibility, polar and nonpolar residue to analyse the regions that are likely exposed on the surface of proteins which are potentially antigenic that allows potential targets to identify the active sites against infection as well as to design effective drug to treat it. Keywords: Dracunculiasis, Antigenic Peptides; MHC-Binders;Tappred;PSSM; SVM; Nonamers; Cytochrome C Oxidase Subunit I (Mitochondrion) I. INTRODUCTION Cytochrome c oxidase subunit I (mitochondrion) comprised of 526 amino acid residues obtained from Dracunculusmedinensisfor the study of MHC class I & II binding peptide, antigenicity, Solvent accessibility, polar and nonpolar residue to analyze the regions that are likely exposed on the surface of proteins.A little dragon from Medina (D.medinesis) is the only species of Dracunculusgenus[1-4] which infects humans, commonly well known as “Guinea worm disease (GWD)”. The other Dracunculus species generally resides in the internal tissues and body cavities of non-human mammals and reptiles (snake and turtles) [5].This nematode undergo a very unusual life cycle of six developmental stages with incubation period last for about one an half years approximately. This is one of the most neglected tropicalparasites which bears clinical importance and needs to be eradicated after small pox [6].After reaching to the maturation stage, these worms copulate and an adult female produces millions of eggs in its uterus whereas mail dies. Later on, the female worm release the larvae which induces a painful blister (1 to 6cm diameter ) on the skin of lower limbs (predominantly localized in the lower extremities(80-90%) in most of the reported cases).The infected person develops slight fever , local skin redness , swelling and severe pruritus around the blister . Other symptoms include: diarrhea, nausea,