5540 Indian Journal of Forensic Medicine & Toxicology, July-September 2021, Vol. 15, No. 3 Effect of Biofertilizer and Spraying Phenylalanine on Mineral Content and Antioxidant Compounds of Dill Thamena Farhan Kadhom Sachet 1 , Maher H. S. Al-Mohammad 2 , Jamal Ahmed Abbass 3 1 PHD Student, Faculty of Agriculture, University of Kufa, Iraq, 2 Professor, College of Agriculture, Al-Qasim Green University, Iraq, 3 Professor, Faculty of Agriculture, University of Kufa, Iraq Abstract The field experiment was carried out in a private field in Babil Governorate, Abi Ghark district, for the winter seasons 2018-2019 and 2019-2020 to study the effect of biological fertilizer and phenylalanine spraying on the content of the shoots and seeds of oil and some active compounds. The treatments were distributed in a global experiment according to a randomized complete block design (RCBD) with three replications. The experiment included two factors, the first is the bacterial vaccine Azotobacter chroococcum with two levels (inoculum and without inoculation) and the second is spraying with the amino acid Phenylalanine in three concentrations 0, 75 and 150 ml -1 . The averages were compared according to the LSD test at a probability level of 0.05. The results showed that the treatment of biological fertilizer and spraying with the amino acid Phenylalanine was significantly superior in all the studied traits, which included the oil yield (gm.plant -1 ), the percentage of nitrogen, phosphorus and potassium and the shoots content of total phenols (mg.kg -1 ) and total flavonoids (mg.kg -1 ) and total flavonoids (mg. . Kg -1 ), percentage of free radical suppression and total antioxidant capacity (mg. 100 g -1 ). The best results were when the two-way treatment (bio-fertilizer×150 ml.1-Phenylalanine), which gave the highest oil yield of 0.677 and 0.907 g. Plant -1 , for the two seasons respectively, compared with the comparison treatment that gave the lowest yield for both seasons of the experiment. Keywords: biological fertilizer, Phenylalanine , Dill(Anethum graveolens L.) Introduction Anethum graveolens L. is an important vegetable plant that belongs to the Apiaceae family and is native to the eastern and western Mediterranean. The active parts are fresh and dry leaves, seeds, roots and oil. It is consumed fresh or cooked and used as a spice and in the manufacture of food and cosmetics, The plant is highly nutritious and rich in vitamins, and contains antioxidants, polyphenols, and other compounds that are considered anticancer, It is used as a treatment for blood pressure, improves the work of the heart and lungs, calms nerves, appetite suppresses, dyspepsia, anti-spasms, vomiting in children, and a diuretic for mothers’ milk (1) . Azotobacter free-living bacteria It is usually spread in light and neutral alkaline soils, it can stabilize atmospheric nitrogen, in addition to its ability to dissolve phosphates and produce various types of growth regulators such as indole acetic acid, gibberellins, cytokinins and a number of vitamins that stimulate the growth of roots and the formation of root hairs. Also, Azotobactere inhibits plant pathogens and the production of cidrofurates (2) . Amino acids are nitrogenous organic compounds that are the basic unit in building proteins, building proteins, and contribute to maintaining the stability of cell acidity. And spraying plants with amino acids contributes to overcoming the nutritional stresses during the plant life cycle (3) as a result of improving the efficiency of absorption and transport of nutrients. As the amino acid Phenylalanine is one of the cyclic (aromatic) amino acids that are used in the construction of many secondary compounds such as alkaloids and vegetable