Open Journal of Applied Sciences, 2018, 8, 141-149 http://www.scirp.org/journal/ojapps ISSN Online: 2165-3925 ISSN Print: 2165-3917 DOI: 10.4236/ojapps.2018.84011 Apr. 24, 2018 141 Open Journal of Applied Sciences Investigating the Effect of Supplementary Feeding on Carbohydrate Composition and Quantity of Royal Jelly Dimitrios Kanelis, Chrysoula Tananaki * , Vasilios Liolios, Maria-Anna Rodopoulou, Georgios Goras, Nikolia Argena, Andreas Thrasyvoulou Laboratory of Apiculture-Sericulture, Aristotle University of Thessaloniki, Aristotle Farm, Thermi, Greece Abstract One of the main practices followed by beekeepers during the production of royal jelly (RJ) is the artificial sugar feeding. In this study, the effect of carbo- hydrate supplementary feeding on the composition of the three main sugars (fructose, glucose, sucrose) and on the final quantity of the product was ex- plored using one-way ANOVA and non-parametric tests. Also, the correla- tions among the parameters were examined. The average yield per colony for non-supplemented colonies (Group A) was 12.8 g, while the average content of fructose, glucose and sucrose was 4.32%, 3.78%, and 0.04%, respectively. For the colonies fed at the grafting day one time (Group B), these values were 12.76 g, 3.11%, 3.19% and 3.71%, and for the colonies fed from the insertion until the collection day (Group C), 12.81 g, 3.05%, 3.12% and 3.54% respec- tively. It should also be noted that the sucrose content in all samples from supplemented colonies was found greater than 1.97%. The statistical tests hig- hlighted the impact of artificial feeding on fructose and glucose contents, while the produced quantity remained uninfluenced. Finally, the Spearman (rho) coefficient test showed statistically significantly negative correlation between the monosaccharides (fructose, glucose) and sucrose. Keywords Royal Jelly, Feeding Experiment, HPLC, Sugars, ANOVA 1. Introduction Royal jelly (RJ), named by the Swiss botanist Francois Hubber in 1788 [1], is a creamy, white and viscous natural secretion from hypopharyngeal and mandi- How to cite this paper: Kanelis, D., Tana- naki, C., Liolios, V., Rodopoulou, M.-A., Goras, G., Argena, N. and Thrasyvoulou, A. (2018) Investigating the Effect of Supple- mentary Feeding on Carbohydrate Compo- sition and Quantity of Royal Jelly. Open Journal of Applied Sciences, 8, 141-149. https://doi.org/10.4236/ojapps.2018.84011 Received: March 7, 2018 Accepted: April 21, 2018 Published: April 24, 2018 Copyright © 2018 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access