This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-Non Commercial- ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. © 2018 Journal of Advanced Pharmacy Education & Research | Published by SPER Publication 9 Validation parameters of densitometric method for simultaneous determination of Galantamine hydrobromide and Pymadine Dobrina Tsvetkova 1* , Stefka Ivanova 1 1 Medical University-Sofia, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 2 Dunav Str., Sofia 1000, Bulgaria Correspondence: Dobrina Tsvetkova, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Sofia, Dunav str. N: 2, 1000, Sofia, Bulgaria. E mail: dobrinka30 @ mail.bg ABSTRACT The aim of the current investigation was the development and validation as per ICH requirements of the TLC-densitometric method with the detection at λ = 282 nm for the simultaneous determination of Galantamine hydrobromide and Pymadine in model mixtures. The aim was in accordance to the perspectives of multi-target therapy of Alzheimer’s disease, and due to the fact that in the literature and pharmacopoeial articles the methods for simultaneous analysis of these components haven’t been described. Selectivity was confirmed by the fact that in blank solution, Rf data weren’t observed corresponding to Rf data of the active ingredients. The regression equations obtained demonstrated the linear relationship between the peak area and concentration: y = 2.10 7 .x + 25364 (Galantamine hydrobromide) (LOD = 1.87.10 -3 g/ml; LOQ = 6.22.10 -3 g/ml); y = 2.10 7 .x + 65930 (Pymadine) (LOD = 2.5.10 -3 g/ml; LOQ = 8.35.10 -3 g/ml). All the experimental data for the degree of recovery were included in the corresponding confidence interval: 93.54 % ÷ 97.32 % (Galantamine hydrobromide); 100.05 % ÷ 103.11 % (Pymadine). The results of precision suited the relevant intervals. System suitability was confirmed by the lack of a statistically significant difference between the Rf values: 0.663 (Galantamine hydrobromide); 0.433 (Pymadine). The method was appropriate for the simultaneous determination of Galantamine hydrobromide and Pymadine in the model mixtures. Keywords: Densitometry, Galantamine, Pymadine, validation, determination. Introduction Alzheimer is a neurodegenerative disease [1] associated with plaques and tangles in the brain [2] . The conventional therapy is with Galantamine (Fig. 1.), Donepezil and Rivastigmine [3] . A potent inhibitory activity against acetylcholinesterase possess the extracts of Ocimum sanctum [4] and Cinnamon zeylanicum [5] . In Alzheimer’s disease [6] , Galantamine improves the behavior [7] by blocking the reversible enzyme acetylcholinesterase and allosterically potentiation of α7-nicotinic acetylcholine receptors [8] , and improves learning and memory alone and in combination with Pymadine [9] . Reactive oxygen species induce neurodegeneration in Alzheimer's disease [10] , and Galantamine is appropriate for the treatmennt due to the antioxidant properties [11] . 4-aminopyridine (Dalfampridine) (Fig. 1.), has been a drug for the symptomatic management of the multiple sclerosis [12] and it improves walking [13] . Figure 1. Chemical structures of Galantamine hydrobromide and 4-aminopyridine. For the analysis of Galantamine in Nivalin, HPLC has been applied [14] . By High Performance Thin Layer Chromatography (HPTLC), Gallic acid, Curcumin and Quercetin are analysed [15] . TLC is often applied for the analysis of plant extracts: Psidium guajava [16] , Nelumbo nucifera [17] , Galantamine in Access this article online Website:www.japer.in E-ISSN: 2249-3379 How to cite this article: Dobrina Tsvetkova, Stefka Ivanova.Validation parameters of densitometric method for simultaneous determination of Galantamine hydrobromide and Pymadine. J Adv Pharm Edu Res 2018;8(4):9- 14. Source of Support: Nil, Conflict of Interest: None declared. Original Article