_____________________________________________________________________________________________________ # PhD Scholar; ≡ Professor; ⱷ Student, M.Sc.; *Corresponding author: E-mail: drujwal1970@gmail.com; Journal of Pharmaceutical Research International 33(64B): 272-279, 2021; Article no.JPRI.80766 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Correlation of MTHFR Gene Polymorphism with Male Infertility Akash More a# , Ujwal Gajbe a*≡ , Bhagyashree Bhandekar bⱷ and Deepti Shrivastava c≡ a Department of Anatomy, Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences, Wardha, India. b Clinical Embryology, Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences, Wardha, India. c Department of Obstetrics and Gynecology, Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences, Wardha, India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i64B35440 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/80766 Received 25 October 2021 Accepted 28 December 2021 Published 30 December 2021 ABSTRACT Background: Folate is present in Vitamin B, which is not present in the body, and one has to consume it from outside. Its functions are to help in the production of RBCs, helps in the production of genes, DNA, RNA, and protein, helps in the metabolism of homocysteine, and is also the main component which is required for the process of methylation. For the metabolism of the folate group, the MTHFR gene is responsible. When there is a problem occurs in the MTHFR gene such as SNP, then it can create a problem with many allied metabolisms and other biological reactions or processes in the body of human body and deficiency of folate in the body may lead to affect various processes such as it can increase the homocysteine level in the body and can cause hyperhomocysteinemia, which has been linked with many diseases along with causing male infertility. Objectives: 1. To identify nucleotide polymorphism in cases and control group. Study Protocol