~1~ International Journal of Herbal Medicine 2016; 4(6): 01-03 E-ISSN: 2321-2187 P-ISSN: 2394-0514 IJHM 2016; 4(6): 01-03 Received: 01-09-2016 Accepted: 02-10-2016 Darshan Singh Department of Chemistry, H. N. B. Garhwal (A Central University), Srinagar Garhwal, Uttarakhand, India Satish C Sati Department of Chemistry, H. N. B. Garhwal (A Central University), Srinagar Garhwal, Uttarakhand, India Maneesha D Sati Department of Chemistry, H. N. B. Garhwal (A Central University), Srinagar Garhwal, Uttarakhand, India Correspondence Darshan Singh Department of Chemistry, H. N. B. Garhwal (A Central University), Srinagar Garhwal, Uttarakhand, India In vitro antimicrobial activity of Himalayan medicinal plant Pholidota articulata Darshan Singh, Satish C Sati and Maneesha D Sati Abstract The antimicrobial activity of the all extracts of Pholidota articulata were studied against five and three fungal stain. The results showed that the minimum inhibitory concentration (MIC) of Pholidota articulata extract was 50μg/ml against Salmonella enterica Typhim The ethyl acetate extract of Pholidota articulata showed significant activity 18±1mm, 16±1mm and 14±1mm against Klebsiella pneumonia Salmonella enterica Typhim and E. coli against food poisoning bacteria and the order of the species based on total antibacterial activity is as follows: Klebsiella pneumonia ˃ Salmonella enterica Typhim ˃ Escherichia coli and phytochemical screening for the presence of glycosides, alkaloids, phenols and tannins. Keywords: Antibacterial, antifungal and phytochemical screening 1. Introduction India has great wealth of medicinal plants and their traditional uses. The use of traditional medicinal plants as a source for relief from illness. Herbal medicine is the oldest form of health care known to mankind. Herbs have been used by all cultures throughout the history and they constitute an integral part of the development of modern civilization. Medicinal and aromatic plants and their derived are rich in antibacterial compounds which could be an alternate way to combat bacterial diseases even against some bacteria which are becoming resistant to certain synthetic medicines. The genus Pholidota (Orchidaceae) belongs to the tribe coelogyneae, and comprises 55 species with a distribution from tropical Asia to tropical Australia and china. Among them 9 species in India. Commonly distributed from submontane to montane Himalaya. The genus pholidota are epiphytic herbs generally grown on rocks and trees [1] . Most plants of the genus P. articulata found in India grow as epiphytes. Some are also found growing on moist, moss covered rock structures on large, hilly slopes .On the earth, out of 4, 22,127 plant species about 35,000 to 70,000 species are used as medicinal plants [2] . In the third world countries, 20,000 plants species are believed to be used medicinally [3] . At present, the pharmaceutical sector in India is making use of 280 medicinal plant species, of which 175 are found in the IHR [4] . The plants of the genus pholidota are used traditionally for medicinal purposes. The whole plant has long been used as a remedy for acute or chronic bronchitis, toothache, treatment of dysentery, infections, asthma, bronchitis, eczema and duodenal ulcer [5] . 2. Materials and Methods 2.1 Plant Material Pholidota articulata (Orchidaceae) whole plants were collected from the Ukhimath, Distt- Rudraprayag, Uttarakhand, in September-October 2014. The plant was authentic and identified by Dr. C. S. Rana, Department of Botany and the voucher specimen number is GUH 4325. H. N. B. Garhwal (A Central University) Srinagar Garhwal, Uttarakhand India. 2.2 Preparation of plant Extract The plant material was separated into its selected part air dried ground to moderately fine powder and soxhlet extracted with increasing polarity solvent (petroleum ether, chloroform, ethyl acetate, acetone, methanolic, ethanolic and water [6] . Each extract was evaporated to dryness under reduce pressure using rotary evaporator. The coarse powder of tuber were subjected to successive hot continuous extraction with various solvent each time before extracting with next solvent the powdered material will be air dried (weight of crude extract 500gm). The various concentrated extracts were stored in air tight container for further studies.