489 Pakistan Veterinary Journal ISSN: 0253-8318 (PRINT), 2074-7764 (ONLINE) Accessible at: www.pvj.com.pk Lipid Lowering Effect of a Herbal Mixture in Hyperlipidaemic Adult Male Albino Mice Ijaz Javed 1 *, Muhammad Sarfraz 1 , Faqir Muhammad 1 , Bilal Aslam 1 , Zia-ur-Rahman 1 , Muhammad Zargham Khan 2 , Tanweer Khaliq 1 , Faqir Hussain Khan 3 and Mahmood Ahmad 4 1 Department of Physiology and Pharmacology 2 Department of Pathology, University of Agriculture Faisalabad; 3 The University of Faisalabad, Faisalabad; 4 Faculty of Pharmacy and Alternative Medicine, Islamia University, Bahawalpur, Pakistan *Corresponding author:sandhu_drijaz@yahoo.com ARTICLE HISTORY (13-241) ABSTRACT Received: Revised: Accepted: July 29, 2013 January 22, 2014 May 15, 2014 The present study was conducted to evaluate the lipid lowering effect of a herbal mixture (containing garlic, lemon, ginger, apple vinegar and honey) in hyperlipidemic adult male albino mice. Animals were divided into six groups. Except normal control group, which was kept on routine mice feed, the rest of the groups were provided with atherogenic diet for 0-15 days (lead-in period) to induce hyperlipidemia. After that period mixture was fed to hyperlipidemic albino mice at the dose level of 1.5, 2.5 and 3.5 ml/kg BW to three treated groups, respectively, for 15-60 days as cellulose replacement in atherogenic diet. Simvastatin (synthetic lipid lowering drug, as reference standard) at the dose rate of 0.6mg/kg BW was fed to the hyperlipidemic albino mice of treated control group for 15-60 days as cellulose replacement in atherogenic diet while untreated control group was kept on atherogenic diet as such. Blood samples were taken and serum was tested for lipid profile parameters at day 0, 15, 30, 45 and 60 after the initiation of experiment. The results suggested that the values of percentage reduction induced after administration of mixture, 3.5 ml/kg and simvastatin, 0.6 mg/kg, respectively, at post treatment day 60, are non-significantly (P>0.05) different (51.63 and 68.61 for TL, 49.61 and 60.26 for TGs, 59.54 and 64.72 for TC and 65.75 and 66.44 for LDL- c. Similarly, respective percentage increase, 20.38 and 26.25, for HDL-c were also mutually non-significant. Therefore, this is concluded that mixture, 3.5 ml/kg and simvastatin, 0.6 mg/kg, are equieffective in treating hyperlipidemia in male albino mice. ©2014 PVJ. All rights reserved Key words: Antihyperlipidemic activity High density lipoprotein Low density lipoprotein Mixture Simvastatin Total cholesterol Total lipids Triglycerides To Cite This Article: Javed I, M Sarfraz, F Muhammad, B Aslam, ZU Rahman, MZ Khan, T Khaliq, FH Khan and M Ahmad, 2014. Lipid lowering effect ofa herbal mixture in hyperlipidemic adult male albino mice. Pak Vet J, 34(4): 489-493. INTRODUCTION About 80% of world’s population depends on indigenous medicinal plants. Sixty one percent of global population has been reported to use herbal therapy in various diseased conditions. Use of indigenous plants as remedy against various diseases is increasing because synthetic drugs possess many side effects (Javed et al., 2009). The epidemiological studies showed that the consumption of fat rich diet and the animal source derived food products are appropriate key factor for coronary heart disease (CHD) and known cause of death, all over the world (Rajendran and Ekambaram, 2010). The risk factors of CHD may include an age, sex, obesity, diabetes, increase in blood cholesterol, triglycerol (TAG), and lipoproteins such as LDL (Nago et al., 2011; Buiet al., 2011). Increased levels of serum total cholesterol, triglycerides, very low density lipoproteins (VLDLs), low density lipoproteins (LDLs) and decreased levels of high density lipoproteins (HDL-c) in blood is referred as hyperlipidemia (Javed et al., 2006). Cardiovascular disease (CVD) is the foremost cause of deaths in South Asia (Lim et al., 2012). South Asians have higher evidence of heart diseases at younger age when compared to developed countries (Prashant et al., 2007). There is certain cholesterol lowering functional foods and extract of traditional plants like Cydonia oblonga Mill which are getting more and more attention these days RESEARCH ARTICLE