_____________________________________________________________________________________________________ † Assistant Professor; # Professor; ‡ Research Scientist; ¥ Associate Professor; *Corresponding author: E-mail: azhaganbio23@gmail.com; Journal of Pharmaceutical Research International 33(59A): 360-367, 2021; Article no.JPRI.79811 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Effect of Enzymatic Changes in Vitamin D Combination with LIV-52 on Carbon Tetrachloride Induced Liver Disease in Wistar Rats K. Ponnazhagan a*† , Ursula Sampson a# , N. Muninathan b‡ , J. Pratheeba a† , B. Sumanth Kumar a¥ and I. Jaiganesh c¥ a Department of Biochemistry, Meenakshi Medical College Hospital and Research Institute, Meenakshi Academy of Higher Education and Research, Enathur, Kanchipuram, Tamilnadu-631552, India. b Central Research Laboratory, Meenakshi Medical College Hospital and Research Institute, Meenakshi Academy of Higher Education and Research, Enathur, Kanchipuram, Tamilnadu-631552, India. c Department of Pediatric and Preventive Dentistry, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research, Alapakkam, Chennai, Tamilnadu-600095, India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i59A34279 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/79811 Received 07 October 2021 Accepted 14 December 2021 Published 16 December 2021 ABSTRACT Aim: The present study was conducted to investigate the hepatoprotective effect of enzymatic changes in vitamin D combination with Liv-52 on CCl 4 induced liver toxicity in Wistar rats. Background: The central organ of liver plays an essential vital role in the metabolism, and the liver is called as the metabolic “engine-room of the body”. Therefore, to maintain a healthy liver is a crucial factor for overall health and well being. The liver is the central organ for pharmaceutical drug or chemicals and xenobiotic detoxification metabolism, which regulates most of medication and Original Research Article