International Journal of Research and Review Vol.7; Issue: 1; January 2020 Website: www.ijrrjournal.com Original Research Article E-ISSN: 2349-9788; P-ISSN: 2454-2237 International Journal of Research and Review (ijrrjournal.com) 345 Vol.7; Issue: 1; January 2020 A Comparative Study of Total Bilirubin, Thyroxine and TSH Levels in Newborn J. Sivakumar 1 , Ursula Sampson 2 , J. Kumar 3 1 Assistant Professor, 2 Professor & HOD, 3 Tutor, Department of Biochemistry, Meenakshi Medical College Hospital & Research Institute, Enathur, Kanchipuram-631502, Tamil Nadu, India. Corresponding Author: J. Sivakumar ABSTRACT The present study was designed to investigate a relationship between total bilirubin levels, Thyroxine and TSH in newborn. Based on the bilirubin levels the study group were grouped as group I and group II. Thyroxine, TSH and total bilirubin of 70 newborn were estimated. The mean serum levels of Total bilirubin, TSH and T4 in group I were 4.7076±1.91mg/dl, 4.44+ 2.62mIU/ml and 1.64±0.41 ng/dl respectively. The mean serum level of total bilirubin, TSH and Thyroxine in Group II were 11.5+ 2.3 mg/dl,4.68+ 3.08mIU/ml,1.66+ 0.51 ng/dl. The P value of group I bilirubin with TSH was 0.0001and P value of group I bilirubin with Thyroxine was 0.09. P value of group I TSH with group II TSH was 0.02. According to the results of this study there is significant correlation between total bilirubin levels, Thyroxine and TSH in newborn. Keywords: Thyroid stimulating hormone (TSH), Thyroxine, Total bilirubin INTRODUCTION In the first week of life Jaundice is one of the clinical signs which is most frequently detected. Hyperbilirubinemia plays an important role in the development of jaundice in 30-50% of term infants and in 80% of pre-term infants. (1,2) Due to insufficient production of thyroid hormones, hypothyroidism results. As a result of congenital hypothyroidism there is a delay in the maturation of hepatic Uridine Diphosphate Glucuronyl Transferase (UDPGT) which results in prolonged conjugated hyperbilirubinemia. (3) Approximately 10% of newborns are seen with hypothyroidism. Due to deficiency in UDGPT activity there is physiological jaundice in newborn. The UDGPT activity increases steadily reaching adult values in 14 weeks. (4,5) The present study has been designed to investigate the relationship between neonatal total bilirubin levels, Thyroxine and TSH in the blood Samples. MATERIAL AND METHODS The study is conducted in department of biochemistry, Meenakshi medical college & hospital Kanchipuram after obtaining the institutional ethical committee clearance.70 Newborns with signs of hyperbilirubinemia were screened for thyroxine, TSH and Total bilirubin levels. Total bilirubin levels were estimated in BS 400 by Grot Jendrassik method. Thyroxine and TSH were measured in chemiluminescence method. Comparison of the levels of bilirubin with Thyroxine and TSH were performed in SPSS V.16 Obtained data were presented as mean, standard deviation (SD). Correlation between total bilirubin and thyroid hormone levels was computed by the Pearson’s correlation coefficient. P<0.05 was considered as significant. INCLUSION CRITERIA Newborn babies of full term gestation with normal weight. EXCLUSION CRITERIA