~ 200 ~ International Journal of Fisheries and Aquatic Studies 2020; 8(6): 200-208 E-ISSN: 2347-5129 P-ISSN: 2394-0506 (ICV-Poland) Impact Value: 5.62 (GIF) Impact Factor: 0.549 IJFAS 2020; 8(6): 200-208 © 2020 IJFAS www.fisheriesjournal.com Received: 22-08-2020 Accepted: 25-10-2020 Haig Babikian PT Asclepius Pharmaceutical Sciences, Indonesia Yusef Babikyan PT Central Proteina Prima, Indonesia Rajeev Kumar Jha PT Asclepius Pharmaceutical Sciences, Indonesia Soy Daniel Wisoyo PT Central Proteina Prima, Indonesia Yuli Asih PT Central Proteina Prima, Indonesia Sarayut Srisombat PT Central Proteina Prima, Indonesia Corresponding Author: Haig Babikian PT Asclepius Pharmaceutical Sciences, Indonesia Performance of natural oil blend formulation (NOBF) against white spot syndrome virus (WSSV) agent in Penaeus vannamei boone, 1931 Haig Babikian, Yusef Babikyan, Rajeev Kumar Jha, Soy Daniel Wisoyo, Yuli Asih and Sarayut Srisombat DOI: https://doi.org/10.22271/fish.2020.v8.i6c.2389 Abstract The white spot syndrome virus (WSSV) is lethal in penaeid shrimp. Successful efforts made to develop a natural oil blend formulation (NOBF) by blending Eucalyptus globulus, Pinus sylvestris, and Lavandula latifolia in different compositions with anti-WSSV properties. A bioassay challenge trial conducted using 1 g of specific pathogen-free Penaeus vannamei Boone, 1931 shrimp in 4 replicates of aquaria for each group. The NOBF dose of 0.4 ppm applied throughout the trial period by mixing in the aquarium water daily, starting seven days before the challenge. The efficacy of NOBF against WSSV was measured using the per os method of a challenge. The cumulative mortality in the positive control group reached 100 % after ten days of the challenge. The NOBF was applied in the commercial shrimp ponds to study its efficacy and palatability. A study in 8 ponds in a WSSV prone area in Indonesia, demonstrated no significant difference in crucial water quality parameters, especially on algae as natural food. The NOBF applied ponds had higher productivity (1.28 Kg of shrimp per m3) as compared to control (1.18 Kg of shrimp per m3). The FCR of NOBF group was lower (1.41) than control (1.53). The survival rate of the NOBF group was higher (86.96%) than control (80.36). The trial outcomes show that NOBF is safe and user-friendly, with properties to reduce pathogen load. Keywords: White spot syndrome viral disease, natural oil, per os challenge, shrimp pond culture, shrimp productivity, natural food Introduction The white spot syndrome virus (WSSV) is the most lethal pathogen of culture shrimp worldwide [1] . It is an enveloped non-occluded DNA virus of the family Nimaviridae under the new genus Whispovirus and is also the most devastating shrimp pathogen ever isolated and studied. This virus is extremely virulent and causes up to 100 % mortality in 3-7 days in all the cultured species of penaeid shrimp [2, 3, 4] . Some successful efforts made previously using natural herbs against shrimp viruses [5, 6, 7, 8] . The plants, such as Eucalyptus globulus, Pinus sylvestris, and Lavandula latifolia are well documented for their anti-viral properties. Eucalyptus extract shows strong anti-viral and anti- bacterial properties [9, 10, 11] . Pinus species have potent anti-viral, anti-bacterial, and immune- modulating properties [12, 13] . The Lavandula have shown strong anti-viral [14] as well as immunomodulatory [15] effects. A successful effort made to blend the oils of similar properties to fight against WSSV more vehemently. The efforts made to test the palatability and its impact on critical water quality parameters in commercial shrimp ponds. Materials and Methods The materials and methods divided into two sections, section 1 as laboratory level trials and section 2 as field trials. Section 1: Laboratory trials NOBF preparation and composition Eucalyptus globulus, Pinus sylvestris and Lavandula latifolia oils obtained from vendors who comply with the strictest industry practices: