Accepted Article Immobilized Lipase Catalyzed Synthesis of n-Amyl Acetate: Parameter Optimization, Heterogeneous Kinetics, Continuous Flow Operation and Reactor Modeling Ashwini C. Mathpati 1 , Saurabh G. Kalghatgi 1 , Channamallikarjun S. Mathpati 2 and Bhalchandra M. Bhanage 1 * 1 Department of Chemistry Institute of Chemical Technology, Matunga, Mumbai, India 400 019 2 Department of Chemical Engineering Institute of Chemical Technology, Matunga, Mumbai, India 400 019 *Corresponding Author E-mail: bm.bhanage@ictmumbai.edu.in Ph. No. 91-22-3361 2603, Fax No. 91-22-3361 1020 Abstract BACKGROUND The work describes synthesis of n-amyl acetate from n-amyl alcohol, by-product of sugar industry. The synthesis was carried out using Burkholderia cepacia lipase immobilized on hydrophobic polymer support containing hydroxypropyl methyl cellulose and polyvinyl alcohol. The reaction conditions were optimized and intrinsic kinetics was evaluated. An immobilized lipase coated film reactor was designed and evaluated for continuous flow synthesis. An attempt has been made to model the coated film reactor based on axial dispersion method. RESULTS The reaction conditions were optimized using central composite design based response surface method. The optimum conditions for a maximum conversion of 99.5% were acyl donor to alcohol ratio 2:1, temperature 50 o C and catalyst loading 40 mg in n-hexane. The intrinsic This article is protected by copyright. All rights reserved. This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/jctb.5645