Research Article A Toxicological Evaluation of a Standardized Hydrogenated Extract of Curcumin (CuroWhiteC) Alastimmanahalli Narasimhiah Ravikumar, 1 Joby Jacob, 2 Sreeraj Gopi , 2 and Tumkur Subbarao Jagannath 1 1 Liveon Biolabs (P) Ltd., Tumkur, Karnataka 572106, India 2 R&D Centre, Aurea Biolabs (P) Ltd., Kolenchery, Cochin, Kerala 682311, India Correspondence should be addressed to Sreeraj Gopi; sreeraj.gopi@plantlipids.com and Tumkur Subbarao Jagannath; jagannath@liveonbiolabs.com Received 12 October 2017; Accepted 19 December 2017; Published 23 January 2018 Academic Editor: Syed Ali Copyright © 2018 Alastimmanahalli Narasimhiah Ravikumar et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A series of toxicological investigations were conducted in order to evaluate the genotoxic potential and repeated-dose oral toxicity of CuroWhite, a proprietary extract of curcumin that has been hydrogenated and standardized to not less than 25% hydrogenated curcuminoid content. All tests were conducted in general accordance with internationally accepted standards. Te test item was not mutagenic in the bacterial reverse mutation test or in vitro mammalian chromosomal aberration test, and no in vivo genotoxic activity was observed in rat bone marrow in the micronucleus test. A 90-day repeated-dose study was conducted in male and female Sprague-Dawley rats. Two mortalities occurred in the main and satellite high-dose groups and were determined due to gavage error. No organ specifc or other toxic efects of the test item were observed up to the maximum dose of 800 mg/kg bw/day, administered by gavage. NOAEL was, therefore, estimated as 800 mg/kg bw/day. 1. Introduction Curcuminoids, which are isolated from turmeric root (Cur- cuma longa Linn.), have a long history of use in the tra- ditional Ayurvedic and Chinese medicines. Curcumin (C1), demethoxycurcumin (C2), and bisdemethoxycurcumin (C3) are the main components in curcumin and are responsible for its biological activities [1]. Curcumin and its structural ana- logues have many biological activities, such as cytoprotection, antioxidant activity, infammatory response modifcation, cardiovascular support, neuroprotection, and radioprotec- tion [2]. Catalytic hydrogenation of curcumin leads to tetrahy- drocurcumin (THC), hexahydrocurcumin (HHC), and octa- hydrocurcumin (OHC) [3]. Tese are the major metabolites of curcumin; like their parent compounds, they have many biological activities [4, 5]. Te efect of THC was studied against ferric nitrilotriacetate- (Fe-NTA-) induced oxidative stress in vivo [6]. THC is more easily absorbed from the gastrointestinal tract than curcumin [6] and induces antioxi- dant enzymes, such as glutathione peroxidase, glutathione S- transferase, and NADPH:quinone reductase, and scavenges Fe-NTA-induced free radicals in vitro better than curcumin. Tese results suggest that curcumin is converted to THC in vivo. Similarly, THC has a higher antioxidant activity than curcumin [7], and the antioxidant activity of THC has been analyzed for its efects on the oxidative modifcation of lipids in vitro. THC showed better antioxidative efects than alpha- tocopherol and curcumin [8]. THC supported normal vascu- lar function in the presence of N-nitro-L-arginine methyl ester hydrochloride in rats, and the efects were associated with the alleviation of oxidative stress [9] while exposure of adenosine diphosphate treated human platelet-rich plasma to HHC resulted in an inhibitory efect on platelet aggregation [10]. Tese results suggest both compounds may have poten- tial to support cardiovascular health. THC was investigated for its possible hepatoprotective efect compared with sily- marin in Wistar rats against erythromycin estolate-induced Hindawi Journal of Toxicology Volume 2018, Article ID 5243617, 16 pages https://doi.org/10.1155/2018/5243617