Research Article Brazilian Red Propolis Induces Apoptosis-Like Cell Death and Decreases Migration Potential in Bladder Cancer Cells Karine Rech Begnini, 1,2 Priscila Marques Moura de Leon, 1,2 Helena Thurow, 1,2 Eduarda Schultze, 1,2 Vinicius Farias Campos, 1,2 Fernanda Martins Rodrigues, 2 Sibele Borsuk, 1 Odir Antônio Dellagostin, 1 Lucielli Savegnago, 1 Mariana Roesch-Ely, 3 Sidnei Moura, 3 Francine F. Padilha, 4 Tiago Collares, 1,2 João Antonio Pêgas Henriques, 3 and Fabiana Kömmling Seixas 1,2 1 Programa de P´ os-Graduac ¸˜ ao em Biotecnologia (PPGB), Biotecnologia/Centro de Desenvolvimento Tecnol´ ogico, Universidade Federal de Pelotas, Campus Universit´ ario s/n, 96010-900 Cap˜ ao do Le˜ ao, RS, Brazil 2 Grupo de Pesquisa em Oncologia Celular e Molecular (GPO), Biotecnologia/Centro de Desenvolvimento Tecnol´ ogico, Universidade Federal de Pelotas, Campus Universit´ ario s/n, 96010-900 Cap˜ ao do Le˜ ao, RS, Brazil 3 Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil 4 Universidade Tiradentes, Aracaju, SE, Brazil Correspondence should be addressed to Fabiana K¨ ommling Seixas; seixas.f@gmail.com Received 7 July 2014; Accepted 6 October 2014; Published 3 November 2014 Academic Editor: Fl´ avia R. F. do Nascimento Copyright © 2014 Karine Rech Begnini et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Natural products continue to be an invaluable resource of anticancer drug discovery in recent years. Propolis is known for its biological activities such as antimicrobial and antitumor efects. Tis study assessed the efects of Brazilian red propolis (BRP) on apoptosis and migration potential in human bladder cancer cells. Te efect of BRP ethanolic extract (25, 50, and 100 g/mL) on 5637 cells was determined by MTT, LIVE/DEAD, and migration (scratch assay) assays. Apoptosis induction was investigated through fow cytometry and gene expression profle was investigated by qRT-PCR. Results showed cytotoxicity on MTT and LIVE/DEAD assays, with IC 50 values of 95 g/mL in 24h of treatment. Cellular migration of 5637 cells was signifcantly inhibited through lower doses of BRP ethanolic extract (25 and 50 g/mL). Flow cytometry analyses showed that BRP induced cytotoxicity through apoptosis-like mechanisms in 5637 cells and qRT-PCR revealed increased levels of Bax/Bcl-2 ratio, p53, AIF, and antioxidant enzymes genes. Data suggest that BRP may be a potential source of drugs to bladder cancer treatment. 1. Introduction Cancer is one of the leading causes of death in both develop- ing and developed countries and is a worldwide concern. A total of 1,660,290 new cancer cases and 580,350 cancer deaths are projected to occur in the United States in 2013 [1] and by 2050, 27 million new cancer cases and 17.5 million cancer deaths are projected to occur in the world [2]. An analysis of the anticancer drugs revealed that 47.1% of the approved anticancer drugs were either unmodifed natural products or their semisynthetic derivatives or synthesized molecules based on natural product compound pharmacophores [3]. Natural products tend to present more structurally diverse “drug-like” and “biologically friendly” molecular qualities than pure synthetic compounds at random [4] and have been considered as an “unlimited” resource for future drug discovery [5]. Propolis is a resinous mixture of substances collected by honey bees (Apis mellifera) from various plant sources. It has been used in folk medicine for centuries mostly due to its antimicrobial and anti-infammatory activities [6]. Notable chemical diferences are ofen found between propolis samples and Brazil has the widest chemical diversity of propolis types [7]. Brazilian red propolis (BRP) is the Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2014, Article ID 639856, 13 pages http://dx.doi.org/10.1155/2014/639856