www.wjpr.net Vol 6, Issue 8, 2017. 1245 Nida et al. World Journal of Pharmaceutical Research THE EFFECT OF ULTRA VIOLET RADIATION ON BACTERIA (STAPHYLOCOCCUS AUREUS AND KLEBSIELLA PNEUMONIAE) Ritu Kela (Ph.D) 1 , Nida Khan (M.Tech)* 2 , Bilqueesa Mushtaq (B.sc) 3 , Mohd. Aziz (B.sc) 4 and Neha Chaudhary (B.sc) 5 1 Department of Bio-Chemistry, College of Applied Education and Health Sciences, Meerut, India. 2 Department of Bio-Technology, College of Applied Education and Health Sciences, Meerut, India. 3,4 Department of Radiology, College of Applied Education and Health Sciences, Meerut, India. 5 Department of Microbiology, College of Applied Education and Health Sciences, Meerut, India. ABSTRACT The present study was aimed at understanding the effect of ultra violet- radiations against bacteria Klebsiella pneumoniae and Staphylococcus aureus. These bacteria were isolated in the laboratory and subsequently sub cultured on NAM media Bacteria Staphylococcus aureus and Klebsiealla pneumoniae were exposed to ultra violet radiations for 5 hours and the effect of ultra violet radiation on bacteria was studied. It was found that ultra violet. – radiated recombinant bacteria when tested with Cefixime (200mg), Oflaxacin (200mg), Ornidazole (200mg), Amoxicillin (200mg), Moxifloxacina (200mg), Ciprofloxacin (200mg). for drug sensitivity showed different levels of resistance against different antibiotic used. KEYWORDS: Radiation, Antibiotics, Recombinant, Bacteria. INTRODUCTION The effect of ultra violet radiation on bacteria has received attention in recent times by many researchers. [1,2,3] Michelle et al., [4] attributed this increasing interest to be as a result of the intractable problem caused by microbial antibiotic resistance. Ritter, et al., [5] were of the World Journal of Pharmaceutical Research SJIF Impact Factor 7.523 Volume 6, Issue 8, 1245-1250. Research Article ISSN 2277– 7105 *Corresponding Author Nida Khan Department of Bio- Technology, College of Applied Education and Health Sciences, Meerut, India. Article Received on 28 May 2017, Revised on 18 June 2017, Accepted on 08 July 2017 DOI: 10.20959/wjpr20178-8969