Quest Journals Journal of Research in Agriculture and Animal Science Volume 9 ~ Issue 9 (2022) pp: 46-50 ISSN(Online) : 2321-9459 www.questjournals.org *Corresponding Author: Sovia Hariani 46 | Page Master in Biotechnology, Postgraduate Andalas University Research Paper Biosecurity Detection on Meat Satay In Padang Barat District, Padang City Sovia Hariani 1 , Endang Purwati 2 1 Master in Biotechnology, Postgraduate Andalas University 2 STIKES Prima Indonesia/ Biotechnology, Postgraduate Andalas University Corresponding Author: Sovia Hariani ABSTRACT:The most common processed meat and cuisine of West Sumatra is satay. Almost all local residents and visitors to West Sumatra, especially the city of Padang, like satay. This study aims to detect the safety and hygiene of beef satay sold in West Padang District, Padang City. This study uses purposive sampling with the criteria; The satay is sold in Padang Barat District, Padang City, made from meat, which has not been and has been roasted, immediately finished a day and stored in the freezer or with added preservatives. Based on the results of biosecurity showed that 13 out of 20 samples were contaminated with E.coli bacteria and there were 2 samples contaminated with Staphylococcus aureus bacteria and no samples were contaminated with Salmonella, sp. In the formalin test, all samples were formalin negative and free of borax. The researcher suggests that meat satay sellers in Padang District, Padang City must always maintain and improve sanitation hygiene in the process of producing and serving satay. KEYWORDS: Biosecurity, Formalin, Hygenic, Satay, Safe Received 02 Sep, 2022; Revised 12 Sep., 2022; Accepted 14 Sep., 2022 © The author(s) 2022. Published with open access at www.questjurnals.org I. INTRODUCTION Law Number 18 the Year 2009 article 58 paragraph 1 states that «To guarantee safe, healthy, intact and halal animal products, the Central Government and Regional Governments following their respective authorities carry out supervision, inspection, testing, standardization, certification, and product registration. animal. » Supervision of the provision of halal food starts from upstream to downstream. One source of food that we usually consume is beef. Beef can be obtained easily in the markets [1]. However, the slaughtering process is still carried out at Slaughterhouses located in each district and city. In producing ASUH meat, two certifications must be owned by RPH, namely the Veterinary Control Number certification issued by the Office that carries out the Veterinary Public Health function in the Province and the halal certification from the MUI [2]. According to the Regulation of the Head of the Food and Drug Supervisory Agency No. 20 of 2019 concerning Supervision of Food Packaging classifies materials that are permitted to be used as Food Packaging into two groups: 1. Food Contact Substance, namely any substance intended to be used as a component of food packaging materials used in the manufacture, packaging, packaging and storage of food, which is in use is not intended to provide technical effects on food (BPOM Regulation No. 20 of 2019 ). 2. Food Contact Materials, namely food packaging materials intended to come into contact with food. includes active food packaging, smart food packaging, adhesives, ceramics, cork, rubber and elastomers, glass, ion exchange resins, metals and metal alloys, paper and cardboard, plastics, regenerated cellulose, silicones, fabrics, waxes, woods, polishes, and coating (BPOM Regulation No. 20 of 2019). Extraordinary events (KLB) of food poisoning in Indonesia for the period 2000-2015, the results of research using a systematic and quantitative study approach state that outbreaks are increasing every year. 60% of food poisoning is thought to be caused by bacteria, without being proven by laboratory test results that the cause of the outbreak was due to bacteria. The highest pathogenic bacteria that cause food poisoning include Escherichia coli, Bacillus cereus, Staphylococcus sp., and Salmonella [3]. Almost all people and visitors in West Sumatra, especially Padang City like satay. Many satay traders can easily be found scattered throughout the city of Padang. The large number of satay sales that have not