Proceeding Manado Health Polytechnic 1 st International Conference. ISSN : 2599-2031 428 Issue 1 Volume 1 December 2017 ANTIDIABETIC EFFECT TEST OF ETHYL ACETATE FRACTION ETHANOL EXTRACT OF ABELMOSCHUS MANIHOT L MEDIK LEAVES IN STREPTOZOTOCIN-INDUCED RATS (Rattusnorvegicus) Elisabeth N. Barung 1 , Selfie P. Ulaen 2 , Yos Banne 3 , Jovie M. Dumanauw 4 , Gledis S. Maluenseng 5 1.2.3.4.5 .Pharmacy Department, Manado Health Polytechnic, Indonesia elisabeth.barung1225@gmail.com ABSTRACT Abelmoschus manihot (L.) Medik is one of the vegetation species commonly consumed by North Sulawesi people as vegetable and is believed useful to cure several diseases, such as cholesterol, hypertension and diabetes. One of the chemical contents of its leaves is flavonoid, widely used to treat diabetes. The aim of this research is to identify the effect of ethyl acetate fraction ethanol extract of Abelmoschus manihot L Medik in Streptozotocin-induced rats (Rattusnorvegicus). This is an experimental research with Pretest and Posttest Control Group Design. Animals used in this research were rats, divided into 5 treatment groups, the negative control, positive control, and groups of ethyl acetate fraction of ethanol extract of Albemoschusmanihot leaves 1.2%, 1.8% and 2.4%. Each group consists of 5 tested rats. Before being treated, the tested rats were induced diabetes by injecting Streptozotocin intraperitonelly. Then, blood sugar levels were measured on the 7th, 14th and 21st day. The results show that fraction of ethyl acetate ethanol extract of Abelmoschus manihot (L.) Medik leaves has antidiabetic effect, signified with the reduction of blood glucose levels in the tested rats. Keywords: Antidiabetic, Ethyl Acetate Fraction, Abelmoschus manihot (L.) Medik INTRODUCTION Based on the data from World Health Organization (WHO), Indonesia ranks the 4th largest number of people with Diabetes Mellitus in the world. The latest estimation of the International Diabetes Federation (IDF), there are 382 million people living with Diabetes in the world in 2013. By 2035, this number is going to increase to 592 million people (Ministry of Health RI, 2014). Diabetes Mellitus disease can be treated with medicinal plants that can overcome Diabetes Mellitus (Sabella, 2010). One of the plants used as a drug for Diabetes is Red Gedi (Abelmoschus manihot (L.) Medical). Red Gedi Plants (Abelmoschus manihot (L.) Medik) is one type of plant that has long been used by people in North Sulawesi (Sulawesi) as vegetable and is believed to have properties to cure some diseases, such as lowering plasma cholesterol levels, cure appendicitis, launching the baby's birth, hypertension and diabetes (Prawira et al, 2015; South et al., 2013). Based on the above background, the researcher is interested to do research on antidiabetic effect test of ethyl acetate