International Journal of Pharmacy and Biological Sciences-IJPBS TM (2021) 11 (1): 131-135 Online ISSN: 2230-7605, Print ISSN: 2321-3272 Research Article | Pharmaceutical Sciences | OA Journal | MCI Approved | Index Copernicus DOI: https://doi.org/10.21276/ijpbs.2021.11.1.17 Kuldeepkumar Sawant* et al www.ijpbs.com or www.ijpbsonline.com 131 UV Spectrophotometric Method Development and Validation of Mesalazine in Bulk and Solid Dosage Form Shrishail Ghurghure 1 , Kuldeepkumar Sawant 1 * and Savita S Deokar 2 1 D.S.T.S Mandal ’s College of Pharmacy, Solapur- 413004, Maharashtra, India. 2 Department of Pharmaceutics, PES, Modern College of Pharmacy, Nigidi, Pune Received: 10 Oct 2020 / Accepted: 8 Nov 2020/ Published online: 01 Jan 2021 *Corresponding Author Email: kuldeepkumarsawant97@gmail.com Abstract Objective: A new, simple, sensitive, precise and reproducible UV spectroscopic method was developed for the estimation of Mesalazine in bulk and Tablet Formulation. Methods: The UV spectrum of Mesalazine in Triflouroacetic acid (0.1%) showed λ max at 300nm. Beer’s law is valid in the concentration range of 10-50 µg/ml. This method was validated for linearity, accuracy, precision, ruggedness, and robustness. Results: The method has demonstrated excellent linearity over the range of 10-50µg/ml with regression equation y = 0.017x + 0.0637 and regression correlation coefficient r 2 = 0.9953. Moreover, the method was found to be extremely sensitive with LOD (5.67 μg/ml) and LOQ (17.19μg/ml). Conclusion: Depending on results the given method can be successfully applied for assay of Mesalazine in tablet formulation. Keywords Mesalazine, UV spectroscopy, method development and validation, Triflouroacetic acid water 0.1%, Tablet Formulation. ***** INTRODUCTION: Mesalazine is also called as mesalamine. It (5‐ aminosalicylic acid) is an anti‐inflammatory agent, structurally related to the salicylates, which is active in inflammatory bowel disease and active ulcerative proctitis. It is a tan to pink crystalline powder, relatively insoluble in chloroform, ether, n‐hexane and ethyl acetate and freely soluble in dil. HCl and alkali hydroxides. Mesalazine is available in tablet dosage forms (400 mg) and is an official drug of USP. The chemical structure of Mesalazine is shown in Figure 1. Fig 1: Chemical Structure of Mesalazine Literature survey reveals that, some study about HPLC determination of Mesalamine and its degradation metabolite in plasma determination of 5‐aminosalicylic acid in pharmaceutical formulation by differential pulse voltammetry. To the best of our knowledge, there is no UV method for the analysis of MSZ in pharmaceutical formulations has been reported in literature survey.