159 Deka et al. Syntheses, Characterization and In-Vitro Anti-Inflammatory Activity of Some Novel Thiophenes Maced J Med Sci. 2012 Jul 31; 5(2):159-163. Macedonian Journal of Medical Sciences. 2012 Jul 31; 5(2):159-163. doi:10.3889/MJMS.1857-5773.2012.0225 Basic Science Syntheses, Characterization and In-Vitro Anti-Inflammatory Activity of Some Novel Thiophenes Satyendra Deka 1* , Shamanna Mohan 2 , Janardhan Saravanan 2 , Manoj Kakati 2 , Apurba Talukdar 1 , Bhargab Jyoti Sahariah 1 , Biplab KumarDey 1 , Rama Kanta Sarma 3 1 Assam Down Town University, Dept. of Pharmacy, Panikhati, Guwahati-26, Assam, India; 2 Department of Pharmaceutical Chemistry, P.E.S. College of Pharmacy, Bangalore-50, Karnataka, India; 3 Govt. Ayurvedic College, Dept. of RS & VK Guwahati-14, Assam, India Citation: Deka S, Mohan S, Saravanan J, Kakati M, Talukdar A, Sahariah BJ, Dey BK, Sarma RK. Syntheses, Characterization and In-Vitro Anti- Inflammatory Activity of Some Novel Thiophenes. Maced J Med Sci. 2012 Jul 31; 5(2):159-163. http://dx.doi.org/10.3889/MJMS.1957- 5773.2012.0225. Key words: Thiophenes; Gewald reaction; in vitro anti-inflammatory activity; Denaturation; Microwave. Correspondence: Mr. Satyendra Deka. Assam Down Town University, Department of Pharmacy, Gandhi Nagar, Panikhaiti, Dist. Kamrup, Guwahati Assam 781026, India. Phone: 0091 9706602852. E-Mail: harekrishnaks@yahoo.com Received: 23-Jan-2012; Accepted: 28-Apr-2012; Online first: 12-Jun-2012 Copyright: © 2012 Deka S. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Competing Interests: The authors have declared that no competing interests exist. Abstract Aim: The present study was aimed to synthesize a series of 2-substituted benzylidine imino-3-(3-chloro- 4-fluorophenyl)-carboxamido-4,5-trimethylene thiophenes SPJ-1(a-m) and to evaluate their in-vitro anti-inflammatory activity. Materials and Methods: The starting material (SPJ-1) was prepared by the application of versatile Gewald reaction. The In-vitro anti-inflammatory activity of synthesized compounds [SPJ-1(a-m)] was evaluated using inhibition of bovine serum albumin denaturation method. Result: SPJ-1b and SPJ-1g have shown significant in-vitro anti-inflammatory activity. Conclusion: The findings of the present study clearly demonstrate that chloro functional group possess inhibition of bovine serum albumin denaturation capacity and has in-vitro anti-inflammatory activity. However hydroxyl, nitro, methyl, methoxy and dimethyl amino derivatives did not show any in-vitro anti- inflammatory activity. OPENACCESS Introduction Thiophene containing organic compounds forms a significant group of drugs which exhibit an array of biological activities ranging from anti-inflammatory [1-5], antibacterial, antifungal, local anaesthetic, analgesic, anti-neoplastic, antiarthritic, antitussive and so on. The starting material 2-amino-3-(3-chloro-4- fluoro phenyl carboxamido)-4,5-trimethylene thiophene (SPJ-1) was prepared by the application of the versatile Gewald reaction [6, 7]. Treatment of starting material with various substituted aromatic aldehydes gave the title compounds SPJ-1(a-m). All the synthesized compounds were characterized by their physical and spectral data. The IR spectra of compound SPJ-1 showed an intense sharp NH 2 absorption peak at 3413.29 cm -2 ; C-S peak at 778.75 cm -2 . The formation of Schiff’s bases SPJ-1(a-m) was confirmed by the presence of an imine ( HC=N)