ISPUB.COM The Internet Journal of Veterinary Medicine Volume 4 Number 1 1 of 4 A Comparative Evaluation of the Activities of LDH and SDH in the Small intestine of Pangolin Manis tricuspis and Bat Eidolon helvum D Ofusori, A Adelakun, O Adesanya, G Ojo, K Oluyemi, K Ajeigbe, A Ajisafe Citation D Ofusori, A Adelakun, O Adesanya, G Ojo, K Oluyemi, K Ajeigbe, A Ajisafe. A Comparative Evaluation of the Activities of LDH and SDH in the Small intestine of Pangolin Manis tricuspis and Bat Eidolon helvum . The Internet Journal of Veterinary Medicine. 2007 Volume 4 Number 1. Abstract The aim of this study was to biochemically investigate the conventional pathways of carbohydrate metabolism adopted by the small intestine of the two mammals in meeting their energy demand with reference to the varied diet, morphometric and histological differences in their intestines. Ten pangolins and ten bats were used for this investigation. The activities of succinate dehydrogenase (SDH) for the kreb citric acid cycle and lactic dehydrogenase (LDH) for the glycolytic pathway were assessed as marker enzymes for carbohydrate metabolism. The results showed that Embden Mayerhoff's pathway is actively utilized by the small intestine of bat for its metabolic processes in contrast to the Krebs citric acid cycle path way in pangolin. This present study had shown that the small intestine of the two mammals utilizes different metabolic pathways in meeting their energy demand. INTRODUCTION A biochemical study of the activities of some enzymes in a particular tissue provide useful information on the state of tissue under normal and diseased conditions and may also reflect some of the metabolic processes presumably occurring within the tissue at the time of the study 1 . Every living cell is known to undergo metabolic processes. This is carried out via different conventional pathways involved in the breakdown of glucose to release energy in the form of ATP which the organisms utilize for its daily activities 2 . It is therefore worth while to comparatively investigate the conventional pathways of carbohydrate metabolism the two mammals (pangolin and bat) have adopted in meeting their energy demand. This is inline with the peculiar diets of the mammals as well as different morphological and morphometric records in some parts of the gastrointestinal tract (GIT). For instance, Ofusori and Caxton-Martins 3 reported that the mucosa of the ileum of the pangolin is modified in to plicae circulares which is absent in bats. Also, Hildebrand and Goslow 4 reported that there is no colon in pangolin unlike the bat. Furthermore, the epithelial lining of the stomach of pangolin was observed to consist of stratified squamous keratinized and simple columnar epithelium in contrast to the simple columnar found in bats 3 . Pangolin and bat are both mammals with different diets and morphological adaptations 4 . Pangolins feed on insects and termites 5 , 6 and commonly found in the south west zone of Nigeria. They are often used as meat because they are rich in protein 7 . Eidolon helvum are fruit eating bats 8 , 9 . They were found in roosting colony at the Obafemi Awolowo University, Nigeria. Their natural posture (up-side-down) makes it a unique mammal 10 , 11 . The small intestine which consisted of the duodenum, jejunum and ileum are very important in the process of digestion and gut motility 12 . The histological view revealed various modification which enables proper function. All these differences have raised more dust as to whether the small intestine in these two mammals could be utilizing the same conventional pathways of carbohydrate metabolism in meeting their energy demand despite lots of anatomical differences they have adopted in coping with their respective diets. MATERIALS AND METHODS CARE OF ANIMALS Ten Pangolin and ten bats were used for this investigation. The pangolins were procured 24 hours prior to sacrifice