Organ-related cigarette smoke-induced oxidative stress is strain-dependent Carlos Romualdo Rueff-Barroso 1 ABCDEF, Eduardo Tavares Lima Trajano 1 BEF, Jackson Nogueira Alves 1 BEF, Rojane Oliveira Paiva 1 AB, Manuella Lanzetti 1 AB, Karla Maria Pereira Pires 1 AB, Frank Silva Bezerra 1 ADEG, Ricardo Aurino Pinho 2 ADEG, Samuel Santos Valenca 1 ADEG, Luis Cristóvão Porto 1 ADEG 1 Laboratory of Tissue Repair, Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, Brazil 2 Exercise Biochemistry and Physiology Laboratory, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Brazil Source of support: This work was supported by grants from FAPERJ and CNPq. ML had a bursary from CAPES. SSV was supported by a Visiting Professor Program of UERJ Summary Background: Cigarette smoke (CS) is associated with oxidative stress in several organs because it contains high concentrations of free radicals and reactive oxygen species. Experimental models, using different strains, provide important insights into the genetic basis of diseases. This study sought to identify, in different mouse strains, the organ that is most-susceptible to CS-induced oxidative stress to ob- tain an optimized experimental animal model of oxidative injury induced by CS. Material/Methods: Male Swiss, DBA/2, C3H, BALB/c, and C57BL/6 mice were exposed to CS 3 times a day (4 ciga- rettes per session) for 60 consecutive days. Control groups from the same strains were sham-treat- ed. Protein content, malondialdehyde level, myeloperoxidase activity, and nitrite level were assayed in lung, liver, kidney, and brain from all strains. Catalase and glutathione peroxidase activities were measured. Analyses of data were done by using a 1-way ANOVA with Bonferroni’s post-test (P<.05). Results: Cigarette smoke exposure resulted in distinct, organ-specific responses among strains. The survival rate of DBA/2 mice was lowest. BALB/c and C57BL/6 strains were more-susceptible to oxidative damage in the lung and liver. C3H and C57BL/6 mice were more-susceptible to oxidative damage in the brain. No renal oxidative damage was seen. Conclusions: Mouse strains and individual organs display a range of susceptibilities to CS-induced oxidative stress. BALB/c and C57BL/6 strains appear to be the best choices as experimental models for studying CS effects on liver and lung, and C3H and C57BL/6 strains for CS-effects on the brain. key words: cigarette smoke • organs • oxidative stress • animal model Full-text PDF: http://www.medscimonit.com/fulltxt.php?ICID=880923 Word count: 3681 Tables: 5 Figures: 3 References: 59 Author’s address: Luis Cristóvão Porto, Laboratório de Reparo Tecidual, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro. Av Prof Manuel de Abreu 444 3o. andar – 20551-170 Rio de Janeiro, RJ, Brazil, e-mail: lcporto@uerj.br or lcmporto@terra.com.br. Authors’ Contribution: A Study Design B Data Collection C Statistical Analysis D Data Interpretation E Manuscript Preparation F Literature Search G Funds Collection Received: 2010.01.19 Accepted: 2010.03.10 Published: 2010.07.01 BR218 Basic Research WWW. MEDSCIMONIT.COM © Med Sci Monit, 2010; 16(7): BR218-226 PMID: 20581770 Current Contents/Clinical Medicine • IF(2009)=1.543 • Index Medicus/MEDLINE • EMBASE/Excerpta Medica • Chemical Abstracts • Index Copernicus