Letter to the Editor Pharmacognosy of plant Acalypha fruticosa Forssk. Acalypha fruticosa. Forssk. is said to be attenuant, alternative, stomachic and alexipharmic. 1 Root and leaf paste is prepared in wa- ter and applied externally to treat skin diseases. 2 This plant extract is reported to be anticancer, antioxidant and anti-inflammatory. 3,4 1. Taxonomy of Acalypha fruticosa Forssk. Domain: Eukaryota Whittaker & Margulis, 1978 – eukaryotes Kingdom: Plantae Haeckel, 1866 Subkingdom: Viridaeplantae Cavalier-Smith, 1981 – Green Plants Phylum: Tracheophyta Sinnott, 1935 Ex Cavalier-Smith, 1998 – Vascular Plants Subphylum: Euphyllophytina Infraphylum: Radiatopses Kenrick & Crane, 1997 Class: Magnoliopsida Brongniart, 1843 – Dicotyledons Subclass: Dilleniidae Takhtajan, 1967 Superorder: Euphorbianae Takhtajan Ex Reveal, 1992 Order: Euphorbiales Lindley, 1833 Family: Euphorbiaceae (yoo-for-bee-AY-see-ee) J.F. Gmelin, 1777, Nom. Cons. Subfamily: Acalyphoideae Tribe: Acalypheae Sub tribe: Acalyphinae Genus: Acalypha (ak-uh-LY-fuh) C. Linnaeus, 1753 Species: Fruticosa Forssk. An aromatic shrub up to 4 m tall (Fig. 1a). Stems pubescent and greenish at first, later glabrescent and reddish-brown. Petioles 0.5– 3 cm longleaf blades 2–7 Â 1–4.5 cm, ovate to rhombic-ovate, shortly caudate-acuminate at the apex, crenate-serrate to dentate on the margin, rounded to wide-cuneate or subtruncate at the base, membranous to thinly chartaceous, sparingly or evenly yellowish-pellucid gland-dotted beneath, sparingly to evenly pu- bescent on both surfaces, and usually more densely so along the midrib and main nerves beneath, 5 (7)-nerved from the base; lateral nerves in 2–4 pairs. Stipules 3–4 mm, narrowly lanceolate, puberulous, chestnut- brown. Plants usually monoecious. Inflorescences rarely exceeding 2 cm in length, spicate, axillary, usually androgynous with a densely congested terminal male portion and with 1–4 bracteate female flowers at or near the base; male bracts 1 mm long, ovate, densely white-pubescent; female bracts foliaceous, accrescent to c. 8–10 Â 10–15 mm, broadly ovate to reniform, crenate or repand-dentate, sparingly yellow gland-dotted and often fairly prominently ribbed on the lower surface, sparingly pubescent, 1-flowered. Male flowers subsessile; buds tetragonous-subglobose, densely pubescent or white-tomentose. Female flowers sessile; sepals 3, 1 mm long, ovate-lanceolate, ciliate; ovary 0.7 mm in diameter, 3-lobed to subglobose, Æsmooth, yellow-glandular in the grooves, densely pubescent; styles 4 mm long, Æfree, laciniate, pink or red. Fruits 2 Â 3 mm, 3-lobed, yellow gland-dotted, evenly pubescent-pilose. Seeds 1.5–2 Â 1–1.3 mm, ellipsoid-ovoid, smooth, brown, with an elliptic vulviform caruncle. A. fruticosa occurs in coastal and de- ciduous bush land and thickets, wooded grassland, riverine grass- land, on rocky shores or outcrops, and in humid localities, from sea-level up to 1400 m altitude. It is common in overgrazed areas. This species is globally distributed in Tropical Africa to India, Myanmar and Sri Lanka. Within India, it is said to be found from Orissa to Tamil Nadu, Karnataka and Kerala. A. fruticosa oc- curs in coastal and deciduous bush land and thickets, wooded grassland, riverine grassland, on rocky shores or outcrops, and in humid localities, from sea-level up to 1400 m altitude. 2. Pharmocognocy – Sirucinni- (Acalypha fruticosa. Forssk.) Fresh plants were collected from Vandavasi, Tamil Nadu, India and identified by Botanist, Siddha Central Research Institute, Chennai – 106. Root, stem, petiole and leaf were fixed in FAA solution (70% ethyl alcohol, formalin and acetic acid in the ratio of 90 ml:5 ml:5 ml). The materials were left in the fluid for three days, after which they were washed in water and dehydrated with tertiary butyl alcohol, paraffin wax was infiltrated and the specimens were embedded in wax for sectioning. Alcoholic safranin (0.5%) counter stained with 0.25% fast green. All slides, after staining in safranin were dehydrated by employing graded series of ethyl alcohol (30%, 50%, 70%, 90% and absolute alcohol) and stained fast green in clove oil and xylol–alcohol (50–50) and passed through xylol and mounted in DPX mountant. Clearing of leaves for studying stomatal number and stomatal index was done by using 5% sodium hydroxide along with chlori- nated soda solution supplemented with gentle heat. Photomicro- graphs were taken with the help of Nikon Eclipse E200 Microscope. Root: Long, unbranched tap root with lateral roots, brown outside and cut surface yellowish, fracture short; no characteristic odour and taste. Stem: Green, erect, branched, pubescent, odour aromatic. Leaf: Leaves ovate–oblong, 25–76 mm long, glandular beneath, pubescent, odour aromatic. 3. Microscopic 3.1. T.S. of root Transverse section of root is circular in outline (Fig. 1b). Epi- blema crushed. Cork is made up of rectangular 10 to 12 layers of Contents lists available at SciVerse ScienceDirect Pharmacognosy Journal journal homepage: www.elsevier.com/locate/phcgj Pharmacognosy Journal 5 (2013) 137–139 0975-3575/$ – see front matter Copyright Ó 2013, Phcog.Net, Published by Reed Elsevier India Pvt. Ltd. All rights reserved. http://dx.doi.org/10.1016/j.phcgj.2013.05.002