_____________________________________________________________________________________________________ *Corresponding author: E-mail: joshineha379@gmail.com; Journal of Pharmaceutical Research International 33(41A): 1-13, 2021; Article no.JPRI.72380 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Development and Evaluation of Nanoemulsions for Phenytoin Drug Loading Neha Joshi 1* , Vijay Juyal 1 , Himanshu Joshi 2 and Shweta Dang 3 1 Department of Pharmaceutical Sciences, Faculty of Technology, Bhimtal Campus, Kumaun University, Nainital, Uttarakhand, 263136, India. 2 College of Pharmacy, Faculty of Technology, Bhimtal Campus, Graphic Era Hill University, Nainital, Uttarakhand, 263136, India. 3 Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, Uttar Pradesh, 201309, India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i41A32297 Editor(s): (1) Dr. Juan Carlos Troiano, University of Buenos Aires, Argentina. Reviewers: (1) Diego da Silva, Uniensino, Brazil. (2) Lilian Elisa Sosa Díaz, National Autonomous University of Honduras, Honduras. (3) Shailesh L Patwekar, SRTM University, India. Complete Peer review History: https://www.sdiarticle4.com/review-history/72380 Received 07 June 2021 Accepted 11 August 2021 Published 14 August 2021 ABSTRACT Aims: To understand about the nanoemulsion types and the process formation of spontaneous emulsification method by phase inversion. Then to test the different combinations of Oil, Surfactants and Co-surfactants for formation of suitable nanoemulsions for phenytoin drug loading. Study Design: Spontaneous emulsification method by phase inversion used to form the nanoemulsions. Place and Duration of Study: Department of Pharmaceutical Sciences, Kumaun University, Nainital, Uttarakhand, India. Methodology: Phenytoin is a widely used drug in anticonvulsants class for epilepsy which comes under BCS Class II of drug category. Phenytoin has high permeability property but it also shows low solubility property which makes it difficult to absorb from GI tract hence make a poor penetration into the brain to target disease in the CNS. To overcome the situation of poor delivery of phenytoin, the requirement of nanoparticulate drug delivery as an innovative and effective drug Original Research Article