How to Cite: Al-Hatemy, M. D. B., & Mohsin, M. I. (2022). Evaluating the levels of interleukin-6 and interleukin-10 as potential biomarkers for the severity and mortality in COVID-19 patients. International Journal of Health Sciences, 6(S8), 294–310. https://doi.org/10.53730/ijhs.v6nS8.9714 International Journal of Health Sciences ISSN 2550-6978 E-ISSN 2550-696X © 2022. Manuscript submitted: 27 March 2022, Manuscript revised: 9 May 2022, Accepted for publication: 18 June 2022 294 Evaluating the levels of interleukin-6 and interleukin-10 as potential biomarkers for the severity and mortality in COVID-19 patients Mohammed Dakhil Bareez Al-Hatemy Al-Najaf health directorate, Department of operations and emergency medicine Corresponding author email: Muhamaddakhil73@gmail.com Muslim Idan Mohsin Department Laboratory Investigation, Faculty of science, University of Kufa Email: Muslim.aljabri@uokufa.edu.iq Abstract---Objective: Evaluating the levels of pro-inflammatory cytokine (IL-6) and anti-inflammatory cytokine (IL-10) among patients with COVID-19. Methodology: A study is conducted during the period from 1 st November 2021 to 1 st April 2022 , 110 patients were diagnosed as COVID-19. Their ages range between 20-81 and more years old, Who attended to AL-Amal Specialized Hospital for Communicable Diseases at first week of infection with symptoms (fever, Headache, cough, shortness of breath, diarrhea, loss of taste and smell), and compared with 50 apparently healthy individuals as control group. The diagnosis of each case was established using clinical diagnosis and confirmed by reverse transcriptase polymerase chain reaction (rt-PCR), 5 ml of fresh venous blood samples were collected from COVID-19 infected patients by sterile syringes and saved in serum separator tube (SST) with specific gel to easy sorting the serum, then left for 20 minutes at room temperature. After coagulation, sera were separated by centrifuge 4000xg for 15 minutes and directly stored at -20 ºC to be analyzed later for IL-6 and IL-10 assay. Results: In the current study, the prevalence of severe COVID- 19 infection according to age are increased in older aged patients, the highest one appeared at the 81 and more age group in 100% followed by (61- 80) age group 92.3%. Seventy diabetic patients are included in this study, 27 (38.6%) of them have severe COVID-19 cases and 31(44.3%) have severe cases with death , the result of statistical analysis demonstrated that statistically significant differences are found in diabetic patients as compared with non-diabetic patients. also the results revealed that there are significant differences in hypertensive patients when compared with non-hypertensive patients (36 vs. 22.9) in severe cases, and (40 vs. 20) in severe cases with