Accepted Preprint This article is protected by copyright. All rights reserved Biofuels and Environmental Biotechnology Biotechnology and Bioengineering DOI 10.1002/bit.26548 Effective Role of Medium Supplementation in Microalgal Lipid Accumulation † Azadeh Fazeli Danesh 1, 2 , Peter Mooij 2 , Sirous Ebrahimi 1,* , Robbert Kleerebezem 2 and Mark van Loosdrecht 2 1 Biotechnology Research Center, Faculty of Chemical Engineering, Sahand University of Technology, Tabriz, Iran 2 Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands * Corresponding author: Sirous Ebrahimi E-mail: sirous.ebrahimi@epfl.ch Tel: +98 41 33458180; Fax: +98 41 33444355 † This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: [10.1002/bit.26548] Additional Supporting Information may be found in the online version of this article. This article is protected by copyright. All rights reserved Received October 17, 2017; Revision Received January 7, 2018; Accepted January 9, 2018