International Journal for Pharmaceutical Research Scholars (IJPRS) V-4, I-2, 2015 ISSN No: 2277 - 7873 RESEARCH ARTICLE © Copyright reserved by IJPRS 475 Effective of Different Carbon Concentration on Yield of Extracellular Polymeric Substances (EPS) Produced by Pseudomonas Putida ATCC 11172 Abubaker K. Elayatt* 1 , Maria E. Romero-Gonzalez 2 1 Department of Chemical Engineering, Engineering Faculty/ Sirte University, Libya. 2 Kroto Research Institute, Department of Civil and Structural Engineering, the University of Sheffield, UK. Manuscript No: IJPRS/V4/I2/00091, Received On: 10/05/2015, Accepted On: 21/05/2015 ABSTRACT The extracellular polymeric substances (EPS) extracted from Pseudomonas putida ATCC 11172 at different carbon concentration, where Luria broth used as medium growth with and without addition of 0.5% w/v (LBG 0.5%) and 1.0% w/v (LBG 1%) glucose. The Bound EPS were extracted by ethylene diamine tetra acetic acid (EDTA) methods and precipitated by cold ethanol 3:1; while free EPS extracted only by precipitate by cold ethanol 3:1. The study shows the clear effect of carbon concentration on the production of EPS. The amount of free EPS found 120.39±2.20, 111.16±1.75 and 100.38±2.0 mg g -1 dry cell for LB, LBG 0.5 and LBG 1 respectively. Where the yield of bound EPS shows fewer amounts from free EPS, the amount of bound EPS was 29.22±1.40, 21.18±0.90 and 17.10±1.10 mg g -1 dry cell for LB, LBG 0.5 and LBG 1 respectively. KEYWORDS Extracellular Polymeric Substances (EPS), Pseudomonas putida ATCC 11172 INTRODUCTION Bacteria secrete extracellular polymeric substances (EPS) to facilitate attachment with each other and to solid surfaces 1,2,3 . EPS is categorized as either free or bound, where the free is released into surrounded media and the bound is attached to cell walls of the bacteria. Cell surfaces interact initially by bound and free EPS at the initial stages of biofilm formation. Extracellular polymeric substances include polysaccharides and proteins as major compounds where the minority is lipids, nucleic acids and other polymers, as will flagella, debris from lysed cells, outer membrane, pili, etc. 4,5,6 . The importance of EPS in the environment is well known. There is great interest in a wide range of applications. e.g. protection of drinking water supply from bacterial contamination 7 ; bioremediation of oil- contaminated environments 8 ; riverbank filtration 9 ; in situ bioremediation of contaminated soil 10 ; in wastewater treatment 11 ; biotechnology used in the food and bioleaching fields due to their wide structural variety 12,13 and biopharmaceutical industries 14 . There is no universal method that can be applied to extract extracellular polymeric substances (EPS) without causing cell lysis 15 . A number of methods have been tested for extracted EPS. Extraction protocols in literature can be divided into three groups: i- physical which consist of the following : centrifuge 16,17,18 , heating 19 , sonication 20 , cationic dowex resin 21,22 , crown ether 23 ; ii- chemical methods: EDTA 19 , NaCl 11 , NaOH 19 , formaldehyde 11 , Sulphuric acid 21,24 , glutaraldehyde 25 ; and iii- a combination of physical and chemical methods of extraction: *Address for Correspondence: Abubaker K. Elayatt Department of Chemical Engineering, Engineering Faculty/ Sirte University, Libya. E-Mail Id: abuknish@hotmail.com