_____________________________________________________________________________________________________ *Corresponding author: E-mail: vikasjjha7@gmail.com; International Research Journal of Pure & Applied Chemistry 23(3): 19-34, 2022; Article no.IRJPAC.89098 ISSN: 2231-3443, NLM ID: 101647669 Exploration of Chemical Composition and Unveiling the Phytopharmaceutical Potentials of Essential Oil from Fossilized Resin of Pinus succinefera Vikas Jha a* , Mohd Aslam Shaikh b , Sakshi Devkar a , Reetikesh Patel a , Shraddha Walunj c , Diksha Rai c , Joshua Koli a , Vrushali Dhamapurkar a , Tisha Jain b , Namrata Jadhav d , Arpita Marick a and Divya Dhopeshwarkar a a National Facility for Biopharmaceuticals, G. N. Khalsa College, Mumbai, Maharashtra, India. b Department of Five Years Integrated Course in Bioanalytical Sciences, GNIRD, G.N. Khalsa College, Matunga-19, Mumbai, Maharashtra, India. c Department of Biotechnology, The Institute of Science, Mumbai, Maharashtra, India. d Department of Biotechnology, Elphinstone College, Mumbai, Maharashtra, India. Authors’ contributions All of the authors mentioned have made substantial, practical and intellectual contributions to the work and have granted permission for it to be published. Article Information DOI: 10.9734/IRJPAC/2022/v23i330464 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/89098 Received 28 April 2022 Accepted 02 July 2022 Published 07 July 2022 ABSTRACT Background: Most of the plant natural flora contributes significantly to medicinal benefits. To address the shortcoming usage of synthetic drugs, natural products are in demand which offer a broad spectrum of activities with negligible side effects. Pinus succinefera is a fossilized resin from coniferous woods that belongs to the genus Pinus of the Pinaceae family and is widely known as Amber. The present study explores the physiochemical properties and broad spectrum of biological activities of the essential oil. Method: The essential oil obtained using the hydro-distillation process, and chemical composition was examined by Gas Chromatography-Mass Spectrometric analysis as well as the functional groups present in the essential oil were determined using Fourier Transform Infrared Spectrometry. Thermal characterisation was performed using the Thermo Gravimetric Analysis and Differential Scanning Calorimetry. The antimicrobial potential of the essential oil was evaluated against different microorganisms meanwhile antimalarial activity against Plasmodium falciparum was Original Research Article