_____________________________________________________________________________________________________ *Corresponding author: E-mail: rsaajani@yahoo.co.uk; Journal of Complementary and Alternative Medical Research 18(3): 43-51, 2022; Article no.JOCAMR.87989 ISSN: 2456-6276 Ocimum gratissimum as a Remedy to Chemical Induced Liver Injury Rotimi Sunday Ajani a* and Gabriel Kehinde Obasa a a Division of Gastrointestinal and Morphological Anatomy, Department of Anatomy, College of Medicine, University of Ibadan, Nigeria. Authors’ contributions This work was carried out in collaboration between both authors. Author RSA conceptualized and designed the study, supervised the experiment, analyzed and interpreted the results and prepared the manuscript. Author GKO performed the experiment, collected the data and performed initial analysis of the data. Authors RSA and GKO did the literature search, read and approved the manuscript. Author RSA is the guarantor of the study. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/JOCAMR/2022/v18i330354 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/87989 Received 09 April 2022 Accepted 18 June 2022 Published 27 June 2022 ABSTRACT Objective: Drug induced liver injury is one of the sequelae of adverse drug reactions. We studied the efficacy of aqueous extract of Ocimum gratissimum at ameliorating carbon tetrachloride induced liver injury. Methodology: The study utilized five groups with six animals each. Liver injury was induced in groups L I (Liver injury), LDE (Low dose extract), MDE (Medium dose extract) and HDE (High dose extract) while group CN served as control. Groups LDE, MDE and HDE respectively had 100 mg/kg, 200 mg/kg and 400 mg/kg of the O.gratissimum aqueous extract daily for twenty one days.Blood samples were thereafter collected for biochemical analyses, the animals sacrificed and harvested livers processed for histopathological evaluation. Results: The mean liver weight of LI was markedly elevated while its total protein level was significantly the least. Extract of O.gratissimum was able to counter the elevated levels of aspartate transaminase and amino transaminase occasioned by the induced liver injury. High dose of O.gratissimum extract restored the activity of glutathione peroxidase hitherto depressed by carbon tetrachloride toxicity. Catalase and super oxide dismutase activities of all the three extract groups were significantly elevated in reference to the LI group. Lipid peroxidation was Original Research Article