Research Article Integrating In Silico and In Vitro Approaches to Screen the Antidiabetic Properties from Tabernaemontana divaricata (Jasmine) Flowers Saima Muzammil , 1 Rahat Andleeb, 2 Sumreen Hayat, 1 Muhammad Umar Ijaz, 3 Asma Ashraf , 2 Nimrah Zafar, 2 Shabana Naz, 2 and Mubashera Shaheen 2 1 Department of Microbiology, Government College University, Faisalabad, Pakistan 2 Department of Zoology, Government College University, Faisalabad, Pakistan 3 Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan Correspondence should be addressed to Asma Ashraf; asmabinm@gmail.com Received 17 January 2022; Revised 28 February 2022; Accepted 17 March 2022; Published 31 May 2022 Academic Editor: Fernanda Tonelli Copyright © 2022 Saima Muzammil et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. e purpose of this study was to assess different in vitro biological activities such as phytochemical constituents, enzymatic antioxidant status, cytotoxicity through hemolytic activity, and antidiabetic potential of plant methanolic extract through glucose uptake by yeast cells. Further, using in silico approach by the SwissADME technique the drug-likeness rules for bioactive components were characterized, while potential interactions were identified via molecular docking of a ligand with target proteins by GOLD 5.3.0. e results showed that T. divaricata was rich in TPC and TFC, i.e., 62.32 ± 4.02 and 24.53 ± 0.61, respectively, and the cytotoxic potential was 10% towards human RBCs, while protein estimation revealed the presence of protein in the extract, which was 22.82 ± 4.6. DPPH assay in comparison with ascorbic acid and several enzymatic assays, such as CAT, SOD, and POD, showed maximum antioxidant potential, i.e.,15.9 ± 2.33%, 65.57 ± 13.4%, 3.02 ± 3.4, 15.87 ± 0.5, and 0.74 ± 0.2, respectively. Glucose uptake by yeast cells, i.e., α-amylase and α-glucosidase, showed a maximum antidiabetic potential such as 75.11 ± 1.44%, 41.81 ± 3.75%, and 35.9 ± 1.24%, respectively. Our results indicate that the methanolic extract of T. divaricata has antioxidant potential and inhibits α-amylase and α-glucosidase activity and possesses maximum antidiabetic potential. e results provide scientific proof that the medicinal plant being studied is a powerful source of natural antioxidant, antidiabetic, and medicinally significant substances. In silico study, using a molecular docking, unveiled that two compounds showed good interactions with 5kzw protein with considerable binding affinities and fulfilled docking parameters. It may conclude that T. divaricata is an important vegetable with a potent source of natural antioxidants and antidiabetic activity justifying its traditional use in green therapeutics. 1. Introduction Diabetes is a metabolic disorder characterized by a high level of glucose in the bloodstream triggered by inadequate in- sulin output or insulin activity [1]. Diabetes is a dynamic chronic disease needing ongoing medical care with harm reduction approaches within glycemic control [2]. Polyuria, polydipsia, dry mouth, itchy skin, blurry vision, nausea, and exhaustion are the typical physical symptoms of DM [3]. Diabetes accounts for over 3.8 million deaths annually and is therefore the fifth leading cause of mortality [4]. is disease is becoming disastrously underdeveloped (low-income) countries [5]. Pakistan is amongst the ten countries that are expected to have high figures of people with diabetes by 2030 [6]. Diabetes increases the risk of several health problems and has a severe effect on the eye, kidney, foot, blood pressure, etc. [2]. α-Amylase involves the starch hydrolysis into the small scraps of sugar [7]. During diabetes, ROS are induced and cause β-cell glucose toxicity [8]. Cytotoxic Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2022, Article ID 4616815, 17 pages https://doi.org/10.1155/2022/4616815