Pharmacologyonline 2: 9961002 (2011) Wei et al. 996 CHARACTERIZATIO OF ATIMICROBIAL, ATIOXIDAT, ATICACER PROPERTIES AD CHEMICAL COMPOSITIO OF MALAYSIA ADROGRAPHIS PAICULATA LEAF EXTRACT Lee Seong Wei 1* , Wendy Wee 2 , Julius Yong Fu Siong 3 and Desy Fitrya Syamsumir 3 1 Department of Agro Industry, Faculty of Agro Industry and Natural Resources Universiti Malaysia Kelantan, Pengkalan Chepa, 16100, Kota Bharu, Kelantan, Malaysia 2 Department of Fisheries Science and Aquaculture Faculty of Agrotechnology and Food Science, Universiti Malaysia Terengganu Kuala Terengganu, 21030, Terengganu, Malaysia 3 Institute of Marine Biotechnology, Universiti Malaysia Terengganu Kuala Terengganu, 21030, Terengganu, Malaysia Summary This study was carried out to characterize antimicrobial and antioxidant activities of Malaysian   leaf extract. The main objective of the present study is to reveal the potential of   leaf to be used as antimicrobial and antioxidant agent for aquaculture feed use. Antimicrobial property of   leaf extract against  ,    ,  sp  sp.,  sp.,  ,     and  . was revealed by using broth micro6dilution method whereas anticancer activity of the extract was determined with Colorimetric MTT (tetrazolium) assay against human breast adenocarcinoma (MCF67). Compounds of the plant extract were screening and identified by using gas chromatography6mass spectrometry (GC6MS). Antioxidant activity of the plant extract was also characterized by using α, α6diphenyl6β6picrylhydrazyl (DPPH) radical scavenging method. The result of the present study showed that   leaf extract possesses antimicrobial, antioxidant and anticancer activities. The minimum inhibitory concentration (MIC) values were ranged from 31.25 to 125 mg/l in which the plant extract was found can inhibit the growth of      sp.,   and   at 31.25 mg/l,    sp,  sp. and   at 62.5 mg/l and it was able to control the growth of and   at 125 mg/l. The plant extract’s antioxidant activity was recorded almost same like Quercetin at the concentration of 10 ppt whereas the value of IC 50 of the plant extract against MCF67 cell was 20.72 ± 1.10 µg/ml. A total of 16 chemical compound were identified (n6hexadecanoic acid, 7.65 %, 9,12,156 Octadecatrienoic acid, methyl ester, (Z,Z,Z)6, 6.58 %, β6sitosterol, 6.35 %, α6D6 Glucopyranoside, methyl, 5.12 %, 9,12,156Octadecatrienoic acid, ethyl ester, (Z,Z,Z)6 5.05 %, 2H616Benzopyran666ol,3,46dihydro62,86dimehtyl626(4,8,126trimethyltridecyl)6 4.20 %, Phytol, 4.17 %, Pentadecanoic acid, 146methyl6, methyl ester, 3.47 %, Methyl 26O6benzyl6β6 D6xylopyranoside, 2.68 %, 9, 126Octadecadienoic acid (Z, Z)6, 2.61 %, 36phenylpropanoic acid, 46hexadecyl ester, 2.48 %, Heneicosanoic acid, 9,126Octadecadienoic acid, methyl ester, (E,E)6, 1.72 %, Oleic acid, 1.72 %, Cholest656en676one, 36fluoro6, (3 β)6, 1.61 %, Glycerin, 1.27 % and 4H6Chromene636carbonitrile, 26amino676benzyloxy646cyclohex636 enyl6, 1.10 %). The findings of this study indicating that methanol extract of   leaf extract possess high antimicrobial and antioxidant properties. Keywords: antioxidant, anticancer, antimicrobial, chemical compound,  