Research Article Anti-Helicobacter pylori, Anti-Inflammatory, Cytotoxic, and Antioxidant Activities of Mace Extracts from Myristica fragrans Naranpraphai Suthisamphat, 1 Bhanuz Dechayont , 1 Pathompong Phuaklee , 1 Onmanee Prajuabjinda, 1 Ratha-Korn Vilaichone, 2 Arunporn Itharat, 1,3 Khwanchanok Mokmued, 4 andNuntikaPrommee 4 1 DepartmentofAppliedaiTraditionalMedicine,FacultyofMedicine,ammasatUniversity,Pathumani12120,ailand 2 Gastroenterology Unit, Department of Medicine, ammasat University, Pathum ani 12120, ailand 3 Center of Excellence in Applied ai Traditional Medicine Research, ammasat University, Pathum ani 12120, ailand 4 Division of Applied ai Traditional Medicine, Faculty of Public Health, Naresuan University, Phitsanulok 65000, ailand Correspondence should be addressed to Bhanuz Dechayont; nuzz@hotmail.com Received 9 January 2020; Accepted 10 March 2020; Published 29 March 2020 Academic Editor: Francesca Borrelli Copyright © 2020 Naranpraphai Suthisamphat et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. e aril (mace) of Myristica fragrans, known as Dok-Chan, is a spice that has long been used for treating stomach dis- comfort, peptic ulcer, and nausea. It is an ingredient in many remedies in ai traditional medicine, e.g., Ya-Hom-ep- Bha-Jit, Ya-Hom-Nao-Wa-Kot, and Ya-at-Bun-Job, which are used to treat dyspepsia and other gastrointestinal tract symptoms. e aqueous and ethanolic extracts of mace were used for all tests. Anti-H.pylori activities were determined by the disc diffusion method and agar dilution. Anti-inflammatory activity was determined by the LPS-induced nitric oxide (NO) inhibition in a RAW264.7 cell line, and cytotoxicity was determined against gastric cancer cell lines (Kato III) using the sulphorhodamine B (SRB) assay. e DPPH radical scavenging and ABTS radical cation decolorization assays were used to determine the antioxidant activities. e result found that the ethanolic extract of mace exhibited antimicrobial activity against H.pylori ATCC 43504 and six clinical strains with MIC values of 125–250 μg/ml. e aqueous extract MICs against H. pylori ATCC reference strain and six clinical strains were 500 μg/ml compared with 0.5 μg/ml for the positive control, clarithromycin. e inhibitory effect of LPS-induced NO release and cytotoxic activity of the ethanolic extract had IC 50 values of 82.19 μg/ml and 26.06 μg/ml, respectively, and the EC 50 values for the DPPH and ABTS antioxidant assays were 13.41 μg/ml and 12.44 μg/ml, respectively. e mace extract also had anticancer properties. In conclusion, the ethanolic mace extract had anti-H. pylori, anti-inflammatory, antioxidant, and anticancer activities. ese data support further preclinical and clinical investigation to see if the mace extract could have a role in treating patients with dyspepsia, peptic ulcers, and possibly gastric cancer. 1.Introduction e common gastrointestinal (GI) conditions include acid reflux, dyspepsia, nausea, vomiting, peptic ulcer, abdominal pain, flatulence, diarrhea, elicobacter pylori (H. pylori) in- fection, and gastrointestinal cancer [1]. Inflammation is the initial immune reaction in response to cells against patho- gens, chemicals, and foreign antigens. NO plays a central role in the regulation of cytokines in the acute inflammatory response and cancer [2]. Increased level of cytokines and oxidants also promote chronic inflammation that can cause DNA damage and the development of cancer [3]. In GI patients, H.pylori infection causes gastritis and gastric ulcer which predispose to gastric cancer [4–6]. Myristica fragrans (MF) is an evergreen tree whose fruit is used to produce mace (aril) and nutmeg. Both are used Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2020, Article ID 7576818, 6 pages https://doi.org/10.1155/2020/7576818