Int.J.Curr.Microbiol.App.Sci (2019) 8(11): 1510-1516 1510 Original Research Article https://doi.org/10.20546/ijcmas.2019.811.175 Effect of Physical and Chemical Induced Mutation on Different Character of Gladiolus (Gladiolus grandiflorus L.) Priyanka Tirkey* and Devi Singh Department of Horticulture (Floriculture and Landscaping), Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj 211007, India *Corresponding author ABSTRACT Introduction Gladiolus (Gladiolus Grandifloras L.) belongs to family Iridaceae and subfamily Ixioidaceae. It is native of South Africa and is attributed as “Queen of bulbous ornamentals” due to its popularity amongst the bulbous ornamentals in the world. Gladiolus is an important cut flower crop which possesses a great potential for export market to European countries especially during winter. In India, gladiolus is commercially grown in west Bengal, Odisha, Uttar Pradesh, Uttarakhand, Tamil Nadu, Punjab, Haryana, Madhya Pradesh, Delhi and International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 11 (2019) Journal homepage: http://www.ijcmas.com Gladiolus „White Prosperity‟, bulbs was induced by gamma rays, Dry Ethyl Methane Sulphonate (DEMS) and soaked Ethyl Methane Sulphonate (SEMS). The treatments consisted of 2 Kr, 4 Kr, 6 Kr, 8 Kr, 10 Kr and 12 Kr of gamma rays; 0.25%, @ 0.5%, @ 0.75%, @ 1.0% and @ 1.25% of DES; 0.25%, @ 0.5%, @ 0.75%, @ 1.0% and @ 1.25% of EMS and control (untreated) in first year. In the second year we take produced corm as seed and observed the changes in different characters of the crop with control seed. Different concentrations of EMS and different level of gamma radiations had a major effect on sprouting and it significantly delayed the emergence of sprouts. About one third decreases in plant height along with reduction in number of leaves per plant and number of cormles per corm was observed in treated plants. In general, the treated population had manifested reduced expression than the control (untreated population) for most of the characters. After the observation it found that maximum plants height was observed in control while minimum height observed in the treatment with Gamma Rays 12Kr while maximum number of leaves and number of shoot per corm were recorded in treatments with Gamma Rays 2Kr and SOAKED EMS @ 0.25%) respectively. Maximum number of spike per plant was recorded in treatments with Soaked EMS @ 0.25% while minimum number of spike per plant was recorded in T 0 (Recommended dose of nutrients through chemical fertilizers). The gladiolus plants could not survive in treatments of gamma rays and its combination with EMS. On the basis of above observations, it may be concluded that 0.25 to 0.50 per cent dry and soaked EMS doses are suitable for inducing mutation in gladiolus. Keywords Gladiolus, Gamma rays mutagenesis, Dry Ethyl Methane Sulphonate (DEMS) Accepted: 12 October 2019 Available Online: 10 November 2019 Article Info