https://www.heighpubs.org/hvsr 0015 https://doi.org/10.29328/journal.ivs.1001030 More Information *Address for Correspondence: Zullyt Zamora Rodriguez, Veterinary Medicine Doctor, PhD and Assistant Professor, Ozonized Products Groups, National Centre for Scientific Research and Veterinary Medicine Faculty, Agrarian University of Havana, Cuba,Tel: 53 53056529, Email: zullyt.zamora@cnic.cu Submitted: February 26, 2021 Approved: March 16, 2021 Published: March 18, 2021 How to cite this article: Rodriguez ZZ, Lemus M, González EF, Lozano OEL. Efficacy of ozonized sunflower oil as treatment of canine generalized demodicosis. Insights Vet Sci. 2021; 5: 015-021. DOI: 10.29328/journal.ivs.1001030 ORCiD: orcid.org/0000-0002-9387-3761 Copyright: © 2021 Rodriguez ZZ, et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Keywords: Ozonized; Sunflower oil; Demodicosis; Dogs; Demodex canis; Treatment; Mite OPEN ACCESS Introduction Demodicosis is a parasitic inϐlammatory skin disease of dogs caused by an excessive proliferation of Demodex canis [1]. A small number of mites may constitute a normal component of the dog’s skin fauna [2], but a proliferation of mites can lead to serious disease. The parasite is not considered contagious except during a few days after birth, when puppies acquire mites through direct skin contact from their mother. Three morphologically different types of Demodex have been Abstract Background: The acaricidal action of Ozonized sunflower oil (OSO) has demonstrated in different clinical cases of different animal species, such as psoroptic rabbits and pig sarcoptic scabies and recently in demodesic goat. Objectives: This study evaluated the effectiveness of OSO as treatment for generalized demodicosis mange in dogs. Animals: Twenty dogs of different breeds, between six months and one year of age with generalized demodicosic mange that attended to Veterinary Clinic “José Luis Callejas” Havana, Cuba, during the year 2015, were included in the study. Methods: The demodicosis diagnostic was based on its clinical history, the mite’s presence by deep scraping and clinical signs. All cases had a history of receiving before conventional treatments without solution. OSO treatment was topically applied daily, every 12 hours, after shaving all affected areas. Monitoring of clinical signs, mite counting on scaling, pruritus and capillary regrowth (7, 14, 28, 56 and 84 days) were performed. Results: The results showed a significant reduction of mite counts, clinical signs and pruritus since the 7 th day of application. The animals recovered the fur on more than 90% of the body surface. All the animals (100%) recovered from generalized demodesic mange in 84 days of treatment. Conclusion: The effectiveness and safety of OSO as election treatment of generalized demodicosis mange in dogs was demonstrated. described and named as species by some authors (D. canis, D. injai, and D. cornei) [3,4]. Canine generalized demodicosis is frequently seen in the clinical practice [5] and is characterized by ϐive or more affected areas or by lesions covering an entire region of the body, and/or pododemodicosis involving two or more paws [6]. The affected areas are erythematous, with comedones, hair loss, follicular papules to pustules and scales. Lymphadenopathy is commonly associated with the disease Research Article Efficacy of ozonized sunflower oil as treatment of canine generalized demodicosis Zullyt Zamora Rodriguez 1 *, Marielys Lemus 2 , Eduardo Fleitas González 3 and Oscar Ernesto Ledea Lozano 4 1 Veterinary Medicine Doctor, PhD and Assistant Professor, Ozonized Products Groups, National Centre for Scientific Research and Veterinary Medicine Faculty, Agrarian University of Havana, Cuba 2 Veterinary Medicine and Zootechnics Doctor, Clinic Laboratory of Veterinary Clinic José Luis Callejas, Habana, Cuba 3 Veterinary Medicine and Zootechnics Doctor, Clinic Veterinary Clinic Almiqui, Habana, Cuba 4 Chemistry, DsC. Department Analytical Chemistry and Pharmaceutical Formulations, Research of Ozonized Products Units, National Centre for Scientific Research, Havana, Cuba