INCIDENCE OF SYSTEMIC AMYLOIDOSIS AND VIABILITY IN A CAPTIVE POPULATION OF CUVIER’S GAZELLES (GAZELLA CUVIERI) L. Salgado Muro * , E. Valderr abano Cano y , A. Ferreira Prado y , J.L. Rois y and M.A. Jim enez Mart  ınez * * Department of Animal Medicine and Surgery, Complutense University of Madrid and y Marcelle Natureza S.L., Lugo, Spain Introduction: Renal amyloidosis has been reported previously in Cuvier’s gazelles (Gazella cuvieri) with implications for survival. The amyloidoses are a group of diseases characterized by the deposition of intracellular or extracellular insoluble protein fibrils. The purpose of this study was to determine retrospectively the presence of amyloid in a captive population of Cuvier’s gazelles, characterize associated lesions, determine the type of amyloid and analyse risk factors for this species. Material and Methods: Eight adult (O9 years of age) Cuvier’s ga- zelles from a zoo in northern Spain died and were subjected to nec- ropsy examination. Samples were processed at the Zoo and Wildlife Pathology Service of the Complutense Veterinary Hospital for histo- pathology, stained with Congo red and processed for electron microscopy. Results: Five gazelles (62.5%) had amyloid in the kidney, liver or spleen. The most affected organ was the kidney. Amyloid deposition was more severe in the medullary interstitium followed by the glomeruli, and was associated with severe interstitial nephritis. The liver contained amyloid along sinusoids and surrounding central veins, with associated hepatocellular atrophy. Splenic amyloidosis was multifocal and predominant in the red pulp. Renal disease was the cause of death in two of these gazelles. The five animals with amyloidosis had underlying chronic inflammatory processes. Conclusions: Our results show a high incidence of systemic, not just renal, amyloidosis in Cuvier’s gazelles, with implications for survival. Given the presence of concurrent inflammation, amyloidosis second- ary to chronic inflammatory disease was presumed. However, a ge- netic/hereditary aetiology was not ruled out given the low genetic variability of these gazelles. LESION PROFILE IN RAPTORS MONITORED DURING THE 2016e2017 H5N8 OUTBREAK IN NORTHERN GERMANY R. Ulrich * , J. Schink€ othe * , O. Krone y and T. Harder z * Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institute, y Leibniz Institute for Zoo and Wildlife Research and z Institute of Diagnostic Virology, Friedrich-Loeffler-Institute, Germany Introduction: During winter 2016e2017, Germany was affected by an outbreak of highly pathogenic avian influenza (HPAI; H5N8 influenza A virus), which caused mass mortality in poultry and various species of wild birds. This study was performed in order to un- ravel the potential thread of H5N8-induced HPAI on raptors. Materials and Methods: Thirty-four raptors (15 white-tailed sea eagles, 14 common buzzards, two northern goshawks, one rough- legged buzzard, one red kite and one peregrine falcon) collected in Northern Germany between 9th November 2016 and 30th March 2017 underwent necropsy examination under biosafety level 3 condi- tions followed by histopathological, immunohistological and virolog- ical investigations. Results: Morphological lesions consistent with HPAI, influenza A vi- rus nucleoprotein-antigen and/or H5- and N8-specific RNAs were de- tected in more than 82% of the raptors, and led to a diagnosis of HPAI as the major reason of disease and/or death. Among the raptors with confirmed HPAIV infection, characteristic gross lesions were scarce and included haemorrhages, typhlitis and myocarditis. In contrast, histopathological findings were common and included en- cephalitis, myocarditis, splenitis and typhlitis. Real-time RT-PCR re- vealed H5- and H8-specific RNAs in brain and/or lungs. Conclusions: Gross lesions are rare in H5N8-induced HPAI in rap- tors and correct diagnosis relies on histopathological, immunohistolog- ical and virological confirmation. The spectrum of wild bird species affected by the 2016e2017 H5N8 virus differs from a previous HPAIV H5N1 epizootic in 2005e2006. In particular, multiple white-tailed sea eagles succumbed to H5N8-induced HPAI. HPAIV H5N8 infection therefore represents an emerging threat for this endangered species. NOVEL SPECIES OF CHLAMYDIA ISOLATED FROM SNAKES ARE TEMPERATURE-SENSITIVE AND EXHIBIT DECREASED SUSCEPTIBILITY TO AZITHROMYCIN E. Staub * , H. Marti * , R. Biondi y , A. Levi y , M. Donati y , H.M.B. Seth-Smith * and N. Borel * * Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Switzerland and y DIMES, Microbiology, Policlinico S. Orsola, University of Bologna, Italy Introduction: Chlamydiae have recently been recognized as emerging pathogens in snakes, although little is known about their growth charac- teristics in vitro. This study aimed to characterize chlamydiae isolated from cloacal and choanal swab samples of different snake species. Materials and Methods: Isolation was attempted on 18 swabs collected from 197 captive snakes originating from different Swiss snake collections. In contrast to previously reported isolation proto- cols, cultures were grown both at 37  C and 28  C. We then investi- gated the morphology, infectivity and growth characteristics of successfully isolated strains (n 5 4) at 28  C and 37  C using immuno- fluorescence and transmission electron microscopy. These isolates were further analyzed by whole-genome sequencing and their in-vitro antibiotic susceptibility was determined in comparison with the hu- man C. pneumoniae strain K6. Results: Isolates from two individual snakes from one farm (isolates A and B) grew better at 37  C, while the cloacal and choanal isolates (C, D) of a snake from another farm grew better at 28  C. All isolates were sensitive to tetracycline and moxifloxacin, although the minimal inhibitory concentration (MIC) of isolate B was slightly higher (0.5e1 mg/ml) compared with the others (0.06e0.125 mg/ml). Inter- estingly, only C. pneumoniae K6 was sensitive to azithromycin, while all snake isolates were intermediate to resistant (2e4 mg/ml). Genomic analysis revealed that the snake isolates represent novel chlamydial species clustering within the Chlamydiaceae family. Conclusions: We report the isolation of snake chlamydiae displaying various novel in-vitro characteristics including temperature sensitivity and decreased susceptibility to azithromycin. INCREASED EXPRESSION OF BASIC TRANSCRIPTION FACTOR-3 IN CANINE LYMPHOMA REVEALED BY TWO- DIMENSIONAL ELECTROPHORESIS A. Kycko * , M. Reichert * , W. qopuszy nski y and A.  Smiech y * Department of Pathology, National Veterinary Research Institute in Pulawy and y Department of Pathological Anatomy, University of Life Sciences in Lublin, Poland Introduction: Proteomic methods allow for identification of poten- tial novel markers of animal diseases. The aim of this study was iden- tification of protein(s) expressed differentially in canine lymphoma by comparative proteomic analysis of fresh frozen neoplastic and non- neoplastic tissues. Materials and Methods: Protein lysates obtained from fresh frozen tissues of canine B-cell lymphomas (n 5 4) and lymph nodes of dogs without lymphoma (n 5 6) were analysed by two-dimensional electro- phoresis (2DE) using gel strips with the pH range 3e10. Spots showing over four-fold differential expression between the two groups were sub- mitted to protein identification by liquid chromatography tandem mass spectrometry. Furthermore, archival paraffin wax-embedded tis- sues of canine lymphomas (n 5 30) and non-neoplastic canine lymph nodes (n 5 7) were analysed by immunohistochemistry for expression of a protein characterised by the highest difference in expression be- tween the two groups of 2D gels; basic transcription factor (BTF)-3. Results: Two spots showed over four-fold increased expression in each lymphoma 2DE gel compared with non-neoplastic samples. In spots of the highest overexpression, BTF-3 was identified. IHC anal- ysis of BTF-3 in canine lymphomas revealed diffuse, mild to moderate cytoplasmic and marginal labelling of cells of neoplastic appearance. In normal lymph nodes cytoplasmic labelling was observed in macro- phages and centroblasts. Conclusions: The proteomic study allowed for identification of BTF- 3 overexpression in canine lymphoma. As indicated by the IHC re- sults, BTF-3 overexpression in 2DE gels was related to the presence of high number of proliferating neoplastic cells expressing the protein. Upregulation of BTF-3, a protein involved in regulation of tumour- associated genes, was previously reported in several human cancers. 114 ESVP, ESTP and ECVP Proceedings 2017 J. Comp. Path. 2018, Vol. 158, 93e149