_____________________________________________________________________________________________________ *Corresponding author: E-mail: mjohnson_2008@yahoo.com, momohjohnson2008@gmail.com; Microbiology Research Journal International 32(7): 7-31, 2022; Article no.MRJI.92461 ISSN: 2456-7043 (Past name: British Microbiology Research Journal, Past ISSN: 2231-0886, NLM ID: 101608140) Evaluation of Secondary Metabolites Profiling of Ginger (Zingiber officinale Roscoe) Rhizome using GC-MS and Its Antibacterial Potential on Staphylococcus aureus and Escherichia coli Johnson Oshiobugie Momoh a* and Oluremi Nurudeen Olaleye b a Biochemistry Unit, Department of Chemical Sciences, College of Basic Sciences, Lagos State University of Science and Technology (LASUSTECH), Ikorodu, Lagos State, Nigeria. b Microbiology Unit, Department of Biological Sciences, College of Basic Sciences, Lagos State University of Science and Technology (LASUSTECH), Ikorodu, Lagos State, Nigeria. Authors’ contributions This work was carried out in collaboration between both authors. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/MRJI/2022/v32i730397 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/92461 Received 24 July 2022 Accepted 30 September 2022 Published 07 October 2022 ABSTRACT Objective: Ginger (Zingiber officinale Roscoe) rhizome is a well-known food spice and flavoring ingredient with wide range of medicinal properties. The rhizome of ginger consists of unique secondary metabolites compounds. The study evaluates the secondary metabolites profiling of ginger (Zingiber officinale Roscoe) rhizome using Gas Chromatography-Mass Spectrometry (GC- MS) and its antibacterial potential on Staphylococcus aureus and Escherichia coli. Methodology: The GC-MS and phytochemical screening of the aqueous ginger ( Zingiber officinale Roscoe) rhizome extract were determined using standard procedures. Antibacterial activities were determined by agar well diffusion methods. The minimum inhibitory concentrations (MIC) were determined using standard procedure. Results: The result of the GC-MS analysis shows that thirty six compounds were identified in the ginger (Zingiber officinale Roscoe) rhizome using GC-MS analysis with tridecane with molecular formula of C 13 H 28 being the most abundant with peak area of 16.94% and retention time of 12.849. The phytochemical screening shows that the plant contains saponins, alkaloids, glycoside, simple Original Research Article