22nd World Congress on Ultrasound in Obstetrics and Gynecology Short oral presentation abstracts which in turn may cause less of a rise in heart rate (HR). Uterine artery Doppler (UtAD) provides a method of assessing risk for the development of pre-eclampsia. We examined the relationship between second trimester UtAD pulsatility index (PI), HR and birth weight. Methods: 99 women were recruited from the high-risk obstetric ultrasound clinic in the second trimester; median (± IQR) age and gestation were 33 (29–37) years and 23+6 (23+3–24+4) weeks respectively. Mean values of UtAD PI were recorded. Cardiovascular measurements including HR were performed at a later date (26+5 (25+6–28+0) weeks gestation). Gender specific z-scores were calculated from the unit’s previous 5 years’ births. Results: We found a significant positive correlation between HR and birth weight z-score, r = 0.22 (P = 0.03, 95% CI: 0.02–0.40). This correlation remained when 4 patients taking β-blockers were excluded, r = 0.21 (P = 0.04, 95% CI: 0.01–0.40). An inverse association was found between UtAD PI and HR r =−0.43 (P = 0.0001, 95% CI: 0.01–0.40). Conclusions: The novel finding is that lower second trimester HR is associated with lower birth weight; we hypothesize that this reflects inadequate cardiovascular adaptation to pregnancy. We confirm the previously described effect of HR on UtAD PI. However, it is not possible to determine whether this was a direct effect of HR on UtAD PI, or whether women with abnormal placentation are more likely to have a slower HR. This has obvious implications for predictive models using both UtAD and HR. Supporting information can be found in the online version of this abstract. OP10.08 Postnatal functional echocardiography (fECHO) in neonates with abnormal fetal umbilical artery Doppler (UAD) R. Olsen 1 , J. Shepherd 2 , D. Pretorius 3 , A. D. Hull 1 , A. Katheria 2 1 Reproductive Medicine, UCSD, San Diego, CA, USA; 2 Pediatrics, UCSD, San Diego, CA, USA; 3 Radiology, UCSD, San Diego, CA, USA Objectives: Abnormal UAD studies are associated with poor neona- tal outcomes. fECHO is used to evaluate postnatal hemodynamic states; a finding of low systemic blood flow is associated with increased neonatal morbidity. We sought to determine if fECHO could identify which fetuses with abnormal UAD were at highest risk of adverse outcomes. Methods: We conducted a retrospective review of fetuses with abnormal UAD who underwent fECHO in the first 72 hours of life because of hemodynamic compromise. Systemic blood flow measurements included right ventricular output (RVO) and superior vena cava (SVC) flow. We calculated the number of days of abnormal UAD prior to delivery, length of NICU stay and age adjusted birth weight percentiles. We noted the occurrence of respiratory distress syndrome (RDS). Results: 59 subjects had abnormal UAD, 15 subjects had both abnormal UAD and fECHO. 5 subjects had abnormal fECHO defined by either low RVO (< 150 ml/kg/min) or low SVC flow (< 50 ml/kg/min). Gestational age at delivery was similar between the two groups. Those with abnormal fECHO had fewer days of abnormal UAD prior to delivery and trended towards greater length of NICU stay. Subjects with abnormal fECHO had higher birth weight percentiles than those with normal fECHO and universally developed RDS. Conclusions: These findings suggest that fECHO may be a useful tool in the postnatal assessment of fetuses with abnormal UAD and that abnormal fECHO may be helpful in predicting postnatal prognosis in this high risk group. OP10.08: Table Normal fECHO (n = 10) Abnormal fECHO (n = 5) P value RVO Flow (ml/kg/min) 250 ± 20 117 ± 26 0.002 SVC Flow (ml/kg/min) 83 ± 4 36 ± 7 0.0001 Gestational age at delivery (wk) 30 ± 0.9 27 ± 1.4 NS Duration of abnormal UAD prior to delivery (days) 29 ± 10 5 ± 2 0.04 Length of NICU stay (days) 59 ± 14 104 ± 29 0.135 Birth Weight Percentile 5 ± 2 25 ± 12 0.05 RDS (%) 40 100 0.02 Values are means ± SE OP10.09 Heat shock protein gene expression in placental insufficiency related pregnancy complications – correlation with severity of the disease and Doppler-determined umbilical artery PI I. Hromadnikova 1 , K. Kotlabova 1 , K. Dlouha 2 , V. Novotna 2 , L. Krofta 2 1 Department of Molecular Biology and Cell Pathology, Charles University, Third Faculty of Medicine, Prague 10, Czech Republic; 2 Institute for the Care of the Mother and Child, Prague, Czech Republic Objectives: We studied expression profile of heat shock proteins (Hsp27, Hsp60, Hsp70, Hsp90 and HspBP1) in placental tissues of 29 normal pregnancies and 38 complicated pregnancies (15 PEP, 8 PEP with IUGR, 7 IUGR, 6 SGA, 2 gestational hypertension). A novel Hsp70 cochaperone called Hsp70 binding protein 1 (HspBP1) is an intracellular protein that inhibits the ability of anti-apoptotic Hsp70 to refold denatured proteins. The data were evaluated with respect to the time of delivery (before and/or after 34 week of gestation), diagnostic criteria for mild and severe pre-eclampsia based on both hypertension and proteinuria and a. umbilicalis PI. Methods: Hsp gene expression was analysed using dual real-time RT-PCR and comparative Ct method. Human Hsp and β-actin primers and probes were designed using Primer Express version 2.0. Results: Expression profile of Hsp90 was significantly higher in intermediate/late PE w or w/o IUGR than in controls (P = 0.003). Interestingly, HspBP1 mRNA levels differentiated between normal pregnancies and those with early PE w or w/o IUGR (P = 0.005) and intermediate/late PE w or w/o IUGR (P = 0.01). HspBP1 gene expression was significantly increased in intermediate/late PE w or w/o IUGR and significantly decreased in early PE w or w/o IUGR when compared to controls. No relation between Hsp gene expression and a umbilicalis PI or severity of the disease was found. Conclusions: These observations suggest that Hsp90 and HspBP1 are aberrantly expressed in placental tissues affected by placental insufficiency. In particular, HspBP1 may represent a new diagnostic marker for both early and intermediate/late placental insufficiency related complications. Further studies are necessary to analyse HspBP1 protein and mRNA levels in maternal circulation to check up if they would reflect pathological changes in placenta. Acknowledgement: This work was supported by SVV 2012/264705. OP10.10 Umbilical artery Doppler reporting in Australasia 2011 E. A. McCarthy 1,2 , A. Shub 1,2 , S. P. Walker 1,2 , G. Paulsen 2 1 Obstetrics and Gynaecology, University of Melbourne, Heidelberg, VIC, Australia; 2 Perinatal Medicine, Mercy Hospital for Women, Heidelberg, VIC, Australia Objectives: To identify which Doppler indices and reference charts are used in Australian and New Zealand obstetric practice as well as scope to improve consistency and chart quality. 86 Ultrasound in Obstetrics & Gynecology 2012; 40 (Suppl. 1): 55–170