Asian Journal of Pharmaceutical Technology & Innovation ISSN: 2347-8810 Research Article Received on: 25-05-2018 Accepted on: 01-06-2018 Published on: 07-06-2018 Corresponding Author: *Dr. Pichapat Piamrojanaphat Department of Biochemistry Faculty of Medicine Chiang Mai University Thailand Phone No.: +66 53 941000 *Email Id phichapat.p@cmu.ac.th www.asianpharmtech.com 22 Ei Ei Hlainget al, Asian Journal of Pharmaceutical Technology & Innovation, 06 (28); 2018; 22-29 Cite this article as: Ei Ei Hlaing, Supicha Rungjarernarreejitt, Puriwat Fakfum, Patipat Kamdenlek, Pichapat Piamrojanaphat, Proteomic analysis to determine mechanism of purple rice bran on pancreatic damage of type 2 diabetic rats, 6(28); 22-38, 2018.. www.asianpharmtech.com Ei Ei Hlaing, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Thailand Ei Ei Hlaing, Department of Biochemistry, University of Medicine, Mandaley, Myanmar Supicha Rungjarernarreejitt, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Thailand Puriwat Fakfum, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Thailand Patipat Kamdenlek, Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Thailand Proteomic analysis to determine mechanism of purple rice bran on pancreatic damage of type 2 diabetic rats Ei Ei Hlaing, Supicha Rungjarernarreejitt, Puriwat Fakfum, Patipat Kamdenlek, Pichapat Piamrojanaphat** ABSTRACT Diabetes is one of the most common metabolic diseases all over the world. Type 2 diabetic contributes nearly 90% of diabetic patients. Type 2 diabetes is characterized by hyperglycemia with dysfunction of pancreatic beta cells and insulin resistance. Anthocyanins are the major functional component in purple rice (Oryza sativa L. indica). Our previous studies showed that supplementation of purple rice husk attenuated hyperglycemia and prevented pancreatic damage. However, the molecular mechanism of anti-diabetes activities of purple rice need more investigated. In this study, pancreatic tissue samples of type 2 diabetic rats with or without purple rice bran supplement were studied through proteomic approach. Five unique proteins were found in purple rice bran supplemented diabetic rats (Rasgrp3, Clca 1, Olr3, Abcc4, Mfn1). These 5 proteins interacted with other proteins functioning in glycolysis (Eno 3) and insulin signaling (Irs 1, Irs 2, Foxo 1, Foxo 3). Through western blot analysis, Glut 2 was restored back in purple rice bran supplementation, indicating the purple rice bran increase glucose uptake of pancreas and increase the release of insulin. Interestingly, glucose transporter 4 (Glut 4) protein were restored back in purple rice bran supplemented diabetic rats via the expression of