the genus Candida in concentrations that ranged from 178.5 e 0.34 mg.mL -1 . Results and Discussion: Physico-chemical properties demonstrated stable cationic nanoparticles (< 160 nm) and homogenous particle size distri- bution (PdI < 0.3), with high peptide EE (> 97%). AFM and FEGSEM images evidenced homogeneous and spherical nanoparticles with smooth surface. The MIC showed 100% visual growth inhibition for Candida albicans (89.2 mg.mL -1 ), C. tropicalis (11.1 mg.mL -1 ) and C. parapsilosis (11.1 mg.mL -1 ) and the results corroborated with MFC, indicating that CN-TistH-Inc clearly presented a fungicidal action. The peptide TistH was used as a control and demonstrated MIC > 178 mg.mL -1 for all the yeast cells tested. In conclu- sion, biodegradable nanocarriers, prepared using simple and reproducible methods, demonstrated the ability to delivery TistH peptide and improve antifungal activity. Acknowledgments: FAPERN, CAPES and CNPq. 076 THE ANALGESIC EFFECT OF CROTALPHINE INVOLVES THE ACTIVATION OF CENTRAL OPIOID AND CANNABINOID RECEPTORS AND THE PARTICIPATION OF MICROGLIA Fl avia Souza Ribeiro Lopes, Aline Carolina Giardini, Morena Brazil Martins Sant’anna, Gisele Picolo. Instituto Butantan, S~ ao Paulo, SP, Brazil Introduction and Objectives: Neuropathic pain is a worldwide public health problem. The usual analgesics used in the clinic do not induce a satisfactory effect in the treatment of this condition, making the search for new analgesic compounds still necessary. Crotalphine (CRP) is a synthetic peptide which induces potent and long-lasting antinociceptive effect in acute and chronic pain models. In relation to its peripheral effect, it is known that it occurs by the release of endogenous opioids, particularly dynorphin A, and this release is dependent on the activation of cannabi- noid receptors. The use of cannabinoids for the relief of symptoms asso- ciated with inflammation and pain has been widely explored. Then, the aims of this project are to evaluate the analgesic effect of CRP on neuro- pathic pain and the mediation of its central effect. Material and Methods: Male C57BL/6 mice were used, provided by the Institutional Animal Care Committee (CEUA nº 3380310317). Neuropathy was induced by partial ligation of the sciatic nerve (PSNL). Mechanical allodynia was evaluated using von Frey filaments, with progressive vari- ation of the pressure applied to the hind paw. Selective antagonists of mu, kappa and delta opioid receptors and CB1 and CB2 cannabinoid receptors, as well as specific antibodies to endogenous opioids met-enkephalin, b- endorphin and dynorphin-A were used. Microglia involvement was eval- uated using minocycline, an inhibitor of this type of cell. Results and Discussion: The results demonstrated that when orally administered, CRO causes partial analgesia during the acute and chronic phases of pain. This analgesic effect, unlike that observed in the periphery, involves the participation of central mu, kappa and delta opioids receptors and also CB1 and CB2 cannabinoid receptors. We also verified that the correspondent endogenous opioid peptides for these receptors are involved in the analgesic effect. Moreover, this effect involves the partic- ipation of glial cells, particularly microglia. Financial support: CAPES, FAPESP (15/01254-1 and CETICS 2013 07467-1). 077 POTENTIAL CHELATOR OF AN ANIONIC PEPTIDE OF THE SCORPION TITYUS STIGMURUS Menilla Maria Alves De Melo, Diana Pontes Da Silva, Ver^ onica S. Oliveira, Daniel L. Pontes, Matheus F. Fernandes-Pedrosa. Universidade Federal Do Rio Grande Do Norte, Natal, RN, Brazil Introduction and Objectives: Anionic Peptides are molecules rich in aspartic acid (Asp) and/or glutamic acid (Glu) residues in the primary structure. The anionic peptides presented, in some species of animals, antimicrobial, immunomodulatory and chelating action of metals. Chelating agents can be used in the therapy of neurodegenerative diseases, cancer treatment, and inflammatory and autoimmune diseases, besides being molecules with potential antioxidant and anticoagulant. Material and Methods: In order to investigate the behavior of anionic peptide of the scorpion T. stigmurus (TanP) in the presence of metallic ions, the present work carried out a study using Uv-Vis spectroscopy and fluorescence to evaluate the reactivity of the peptide to bivalent metallic ions of biological importance, such as Cu (II), Ca (II), Fe (II) and Zn (II). Molecular dynamic (MD) simulations were performed to show the bond between copper and TanP. Results and Discussion: The UV-vis spectra showed that the Cu (II) and Fe (II) had a significant interaction with the TanP, with a molar ratio of 1: 3 (TanP: Cu 2+ ) and 1: 5 (TanP: Fe 2+ ). For Zn (II) and Ca (II), no relevant spectral change was observed. The fluorescence emission spectra of TanP were obtained with an addition of iron (II) and zinc (II), showing relevant spectral variation only in the presence of iron (II), obtaining a ratio of 1: 7.4 (TanP: Fe 2+ ). TanP showed an intense emission band with a ʎ m ax around 353 nm when excited at 285 nm, which is in accordance with the tryp- tophan emission range. Therefore, it was verified that all results corrobo- rate to show the interaction of the TanP with the metal center. Altogether, the results suggest TanP is a promising peptide for therapeutic and biotechnological application as a chelating agent. 078 SNAKE VENOM ACTIVITIES AS A TOOL TO DEVELOP NEW METALLOPROTEINASE INHIBITORS FROM SYNTHETIC DERIVATIVES OF LAPACHOL Marcelo Abrah~ ao Strauch 1 , Paulo De Assis Melo 2 , Marcos Monteiro Machado 3 , Fernando Chagas Patrao-Neto 3 , Pamella Dourila Nogueira- Souza 3 , Jhonatha Da Mota Teixeira-Cruz 3 , Sara L.S. Gomes 4 , Paulo Roberto Ribeiro Costa 5 , Edgar Schaeffer 5 , Alcides Jose Monteiro Da Silva 5 , Paulo A. Melo 3 . 1 Instituto Vital Brazil, Niter oi, RJ, Brazil; 2 Laborat orio De Farmacologia Das Toxinas, CCS, UFRJRJ, Rio De Janeiro, RJ, Brazil; 3 Laborat orio De Farmacologia Das Toxinas, CCS, UFRJ, Rio De Janeiro, RJ, Brazil; 4 Instituto De Pesquisas De Produtos Naturais Walter Mors, Universidade Federal Do Rio De Janeiro, Rio, Rio De Janeiro, RJ, Brazil; 5 Instituto De Pesquisas De Produtos Naturais Walter Mors, Universidade Federal Do Rio De Janeiro, Rio De Janeiro, RJ, Brazil Introduction and Objectives: It is known that local tissue injuries incurred by snakebites are quickly instilled causing extensive, irreversible, severe tissue destruction. Such injuries are not completely neutralized by the available antivenins, which in general are focused on halting systemic effects. We have used the Bothrops asper venom activities to identify, select and design and synthetize a compound a quinone derivatives of lapachol that could inhibit it’s hemorrhagic and proteolytic activities. Material and Methods: We tested the ability of some new potential active analogues based on the 2-hydroxi-naphthoquinone scaffold to antagonize important activities of Bothrops atrox venom, under different experimental protocols and bioassays either in vitro or in vivo. We investigated the venom-induced hemorrhage, edematogenic, and myotoxic effects in mice in vivo as well as procoagulant, phospholipase A 2 , collagenase and pro- teolytic activities in vitro. Results and Discussion: Proteolytic and collagenase activities of Bothrops atrox venom were shown to be inhibited by lapachol analogues named 3a, 3b, 3c, 3e. The inhibition of these enzymatic activities might help to explain the effects of the analogue 3a in vivo, which decreased skin hemorrhage induced by the venom. The analogues 3a and 3b did not inhibit the myotoxic activity and partially inhibited, less than 20% the phospholipase A 2 induced by Bothrops atrox venom. The lack of protective effect of these compounds against the myotoxicity can be partially explained by their lack of ability to effectively inhibit phospholipase A 2 venom activity. Bothrops atrox venom also induced edema, which was significantly reduced by the analogue 3a which is especially a metal- loproteinase inhibitor of collagenase and proteolytic activities in vitro. 079 EVALUATION OF SELF-ASSEMBLED BOTHROPS JARARACUSSU SNAKE VENOM PROTEINS CROSS-LINKED CHITOSAN NANOPARTICLES ACTIVITY FOR USE AS A POTENTIAL ANTIBACTERIAL Anne Caroline Ribeiro Bastos De França, Karla Samara Rocha Soares, Fiamma Gl aucia Da Silva, Alessandra Danielle Da Silva, Manoela Torres Do R^ ego, Diana Pontes Da Silva, Sarah De Sousa Ferreira, Allanny Alves Furtado, Arn obio Ant^ onio Da Silva Júnior, Matheus De Freitas Abstracts / Toxicon 168 (2019) S1eS42 S32